Gene description for Ogdh
Gene name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Gene symbol Ogdh
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Ogdh
ExoCarta ExoCarta_360975
Vesiclepedia VP_360975
Entrez Gene 360975
UniProt Q5XI78  
 Ogdh identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ogdh
Molecular Function
    oxoglutarate dehydrogenase (succinyl-transferring) activity GO:0004591 IBA
    oxoglutarate dehydrogenase (succinyl-transferring) activity GO:0004591 IDA
    oxoglutarate dehydrogenase (succinyl-transferring) activity GO:0004591 IEA
    oxoglutarate dehydrogenase (succinyl-transferring) activity GO:0004591 ISO
    oxoglutarate dehydrogenase (succinyl-transferring) activity GO:0004591 ISS
    oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor GO:0016624 IEA
    thiamine pyrophosphate binding GO:0030976 IEA
    thiamine pyrophosphate binding GO:0030976 ISO
    thiamine pyrophosphate binding GO:0030976 ISS
    heat shock protein binding GO:0031072 IPI
    oxoglutarate dehydrogenase (NAD+) activity GO:0034602 IEA
    oxoglutarate dehydrogenase (NAD+) activity GO:0034602 ISO
    metal ion binding GO:0046872 IEA
    protein-folding chaperone binding GO:0051087 IPI
Biological Process
    generation of precursor metabolites and energy GO:0006091 ISS
    glycolytic process GO:0006096 IEA
    tricarboxylic acid cycle GO:0006099 IBA
    tricarboxylic acid cycle GO:0006099 IDA
    2-oxoglutarate metabolic process GO:0006103 IDA
    2-oxoglutarate metabolic process GO:0006103 IEA
    2-oxoglutarate metabolic process GO:0006103 ISO
    succinyl-CoA metabolic process GO:0006104 IDA
    succinyl-CoA metabolic process GO:0006104 IEA
    succinyl-CoA metabolic process GO:0006104 ISO
    NADH metabolic process GO:0006734 IDA
    cerebellar cortex development GO:0021695 IEA
    cerebellar cortex development GO:0021695 ISO
    striatum development GO:0021756 IEA
    striatum development GO:0021756 ISO
    hippocampus development GO:0021766 IEA
    hippocampus development GO:0021766 ISO
    thalamus development GO:0021794 IEA
    thalamus development GO:0021794 ISO
    pyramidal neuron development GO:0021860 IEA
    pyramidal neuron development GO:0021860 ISO
    tangential migration from the subventricular zone to the olfactory bulb GO:0022028 IEA
    tangential migration from the subventricular zone to the olfactory bulb GO:0022028 ISO
    olfactory bulb mitral cell layer development GO:0061034 IEA
    olfactory bulb mitral cell layer development GO:0061034 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    mitochondrial membrane GO:0031966 ISS
    oxoglutarate dehydrogenase complex GO:0045252 IBA
    oxoglutarate dehydrogenase complex GO:0045252 IDA
    oxoglutarate dehydrogenase complex GO:0045252 IEA
    oxoglutarate dehydrogenase complex GO:0045252 ISO
 Experiment description of studies that identified Ogdh in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ogdh
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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