ExoCarta: Gene summary

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Gene description for Stk24
Gene name	serine/threonine kinase 24
Gene symbol	Stk24
Other names/aliases	MST-3 Mst3b RGD1561742
Species	Rattus norvegicus
Database cross references - Stk24
ExoCarta	ExoCarta_361092
Entrez Gene	361092
UniProt	B0LT89
Stk24 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Stk24

Molecular Function
	metal ion binding	GO:0046872	IEA
	protein serine/threonine kinase activity	GO:0004674	ISS
	ATP binding	GO:0005524	IEA
	molecular_function	GO:0003674	ND
Biological Process
	execution phase of apoptosis	GO:0097194	ISO
	regulation of apoptotic process	GO:0042981	IBA
	positive regulation of axon regeneration	GO:0048680	IMP
	activation of protein kinase activity	GO:0032147	IBA
	protein autophosphorylation	GO:0046777	ISS
	signal transduction by protein phosphorylation	GO:0023014	IBA
	protein phosphorylation	GO:0006468	ISS
	negative regulation of cell migration	GO:0030336	ISS
	response to hydrogen peroxide	GO:0042542	ISO
	regulation of mitotic cell cycle	GO:0007346	IBA
	regulation of axon regeneration	GO:0048679	ISO
	intrinsic apoptotic signaling pathway in response to oxidative stress	GO:0008631	ISS
	stress-activated protein kinase signaling cascade	GO:0031098	IBA
Subcellular Localization
	cytoplasm	GO:0005737	ISS
	extracellular exosome	GO:0070062	ISO
	Golgi membrane	GO:0000139	IDA
	nucleus	GO:0005634	ISS
	cellular_component	GO:0005575	ND
	nucleolus	GO:0005730	ISO

Experiment description of studies that identified Stk24 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D7 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.25 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting