Gene description for Cul4a
Gene name cullin 4A
Gene symbol Cul4a
Other names/aliases RGD1563853
Species Rattus norvegicus
 Database cross references - Cul4a
ExoCarta ExoCarta_361181
Entrez Gene 361181
 Cul4a identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Cul4a
Molecular Function
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    regulation of protein metabolic process GO:0051246 ISO
    negative regulation of granulocyte differentiation GO:0030853 ISO
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 ISO
    somatic stem cell maintenance GO:0035019 ISO
    hemopoiesis GO:0030097 ISO
    regulation of nucleotide-excision repair GO:2000819 ISO
    regulation of DNA damage checkpoint GO:2000001 ISO
    positive regulation of cell proliferation GO:0008284 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
Subcellular Localization
    Cul4A-RING E3 ubiquitin ligase complex GO:0031464 ISO
    Cul4-RING E3 ubiquitin ligase complex GO:0080008 ISO
 Experiment description of studies that identified Cul4a in exosomes
1
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Cul4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Cul4a is involved
No pathways found





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