Gene description for Fech
Gene name ferrochelatase
Gene symbol Fech
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Fech
ExoCarta ExoCarta_361338
Vesiclepedia VP_361338
Entrez Gene 361338
 Fech identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Fech
Molecular Function
    ferrochelatase activity GO:0004325 IBA
    ferrochelatase activity GO:0004325 IDA
    ferrochelatase activity GO:0004325 ISO
    iron ion binding GO:0005506 ISO
    heme binding GO:0020037 ISO
    iron-responsive element binding GO:0030350 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    tetrapyrrole binding GO:0046906 ISO
Biological Process
    porphyrin-containing compound biosynthetic process GO:0006779 ISO
    heme biosynthetic process GO:0006783 IBA
    heme biosynthetic process GO:0006783 IDA
    heme biosynthetic process GO:0006783 ISO
    heme biosynthetic process GO:0006783 ISO
    heme A biosynthetic process GO:0006784 ISO
    heme B biosynthetic process GO:0006785 ISO
    cholesterol metabolic process GO:0008203 ISO
    response to xenobiotic stimulus GO:0009410 IEP
    response to light stimulus GO:0009416 ISO
    detection of UV GO:0009589 ISO
    response to metal ion GO:0010038 IEP
    response to lead ion GO:0010288 IEP
    regulation of eIF2 alpha phosphorylation by heme GO:0010999 ISO
    response to insecticide GO:0017085 IEP
    erythrocyte differentiation GO:0030218 ISO
    very-low-density lipoprotein particle assembly GO:0034379 ISO
    heme metabolic process GO:0042168 TAS
    response to ethanol GO:0045471 IEP
    protoporphyrinogen IX metabolic process GO:0046501 ISO
    protoporphyrinogen IX metabolic process GO:0046501 ISO
    response to arsenic-containing substance GO:0046685 IEP
    regulation of hemoglobin biosynthetic process GO:0046984 ISO
    heme O biosynthetic process GO:0048034 ISO
    response to methylmercury GO:0051597 IEP
    multicellular organismal-level iron ion homeostasis GO:0060586 ISO
    response to platinum ion GO:0070541 IEP
    cellular response to dexamethasone stimulus GO:0071549 IEP
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial matrix GO:0005759 ISO
 Experiment description of studies that identified Fech in sEVs
1
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Fech
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Fech is involved
No pathways found





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