Gene description for Lcmt1
Gene name leucine carboxyl methyltransferase 1
Gene symbol Lcmt1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Lcmt1
ExoCarta ExoCarta_361643
Vesiclepedia VP_361643
Entrez Gene 361643
UniProt Q6P4Z6  
 Lcmt1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Lcmt1
Molecular Function
    protein C-terminal carboxyl O-methyltransferase activity GO:0003880 ISO
    methyltransferase activity GO:0008168 IEA
    protein methyltransferase activity GO:0008276 ISO
    protein C-terminal leucine carboxyl O-methyltransferase activity GO:0018423 IBA
    protein C-terminal leucine carboxyl O-methyltransferase activity GO:0018423 IEA
Biological Process
    protein methylation GO:0006479 ISO
    C-terminal protein methylation GO:0006481 ISO
    regulation of glucose metabolic process GO:0010906 IEA
    regulation of glucose metabolic process GO:0010906 ISO
    negative regulation of protein-containing complex assembly GO:0031333 IEA
    negative regulation of protein-containing complex assembly GO:0031333 ISO
    methylation GO:0032259 IEA
    regulation of apoptotic process GO:0042981 IEA
    regulation of apoptotic process GO:0042981 ISO
    regulation of mitotic cell cycle spindle assembly checkpoint GO:0090266 IBA
    regulation of mitotic cell cycle spindle assembly checkpoint GO:0090266 IEA
    regulation of mitotic cell cycle spindle assembly checkpoint GO:0090266 ISO
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Lcmt1 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Lcmt1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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