Gene description for Nap1l4
Gene name nucleosome assembly protein 1-like 4
Gene symbol Nap1l4
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Nap1l4
ExoCarta ExoCarta_361684
Vesiclepedia VP_361684
Entrez Gene 361684
UniProt Q5U2Z3  
 Nap1l4 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Nap1l4
Molecular Function
    chromatin binding GO:0003682 IBA
    nucleosome binding GO:0031491 IEA
    nucleosome binding GO:0031491 ISO
    nucleosome binding GO:0031491 ISS
    histone binding GO:0042393 IBA
Biological Process
    nucleosome assembly GO:0006334 IBA
    nucleosome assembly GO:0006334 IEA
    nucleosome assembly GO:0006334 ISO
    nucleosome assembly GO:0006334 ISS
Subcellular Localization
    chromatin GO:0000785 IBA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    dendrite GO:0030425 IDA
    neuronal cell body GO:0043025 IDA
 Experiment description of studies that identified Nap1l4 in sEVs
1
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Nap1l4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mib1  
Affinity Capture-MS Rattus norvegicus
2 MTNR1B  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which Nap1l4 is involved
No pathways found





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