Gene description for Cyb561a3
Gene name cytochrome b561 family, member A3
Gene symbol Cyb561a3
Other names/aliases Cybasc3
RGD1304670
Species Rattus norvegicus
 Database cross references - Cyb561a3
ExoCarta ExoCarta_361729
Vesiclepedia VP_361729
Entrez Gene 361729
UniProt Q5U2W7  
 Cyb561a3 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Cyb561a3
Molecular Function
    oxidoreductase activity GO:0016491 IBA
    oxidoreductase activity GO:0016491 IEA
    metal ion binding GO:0046872 IEA
    transmembrane ascorbate ferrireductase activity GO:0140571 IEA
    transmembrane ascorbate ferrireductase activity GO:0140571 ISO
    transmembrane ascorbate ferrireductase activity GO:0140571 ISS
Biological Process
    intracellular iron ion homeostasis GO:0006879 IEA
    intracellular iron ion homeostasis GO:0006879 ISO
    intracellular iron ion homeostasis GO:0006879 ISS
    transmembrane transport GO:0055085 IEA
Subcellular Localization
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    lysosome GO:0005764 ISO
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IEA
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    membrane GO:0016020 IEA
    late endosome membrane GO:0031902 IEA
    late endosome membrane GO:0031902 ISO
    late endosome membrane GO:0031902 ISS
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Cyb561a3 in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Cyb561a3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Cyb561a3 is involved
No pathways found





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