Gene description for Tkfc
Gene name triokinase/FMN cyclase
Gene symbol Tkfc
Other names/aliases Dak
RGD1311026
Species Rattus norvegicus
 Database cross references - Tkfc
ExoCarta ExoCarta_361730
Entrez Gene 361730
UniProt Q4KLZ6  
 Tkfc identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Tkfc
Molecular Function
    ATP binding GO:0005524 IEA
    triokinase activity GO:0050354 ISO
    glycerone kinase activity GO:0004371 ISO
    FAD-AMP lyase (cyclizing) activity GO:0034012 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    regulation of innate immune response GO:0045088 ISO
    glycerol metabolic process GO:0006071 IEA
    carbohydrate phosphorylation GO:0046835 ISO
    cellular carbohydrate metabolic process GO:0044262 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Tkfc in exosomes
1
Experiment ID 95
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tkfc
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tkfc is involved
PathwayEvidenceSource
Fructose catabolism IEA Reactome





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