Gene description for Tkfc
Gene name triokinase/FMN cyclase
Gene symbol Tkfc
Other names/aliases Dak
RGD1311026
Species Rattus norvegicus
 Database cross references - Tkfc
ExoCarta ExoCarta_361730
Vesiclepedia VP_361730
Entrez Gene 361730
UniProt Q4KLZ6  
 Tkfc identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Tkfc
Molecular Function
    glycerone kinase activity GO:0004371 IBA
    glycerone kinase activity GO:0004371 IEA
    glycerone kinase activity GO:0004371 ISO
    ATP binding GO:0005524 IEA
    FAD-AMP lyase (cyclizing) activity GO:0034012 IEA
    FAD-AMP lyase (cyclizing) activity GO:0034012 ISO
    metal ion binding GO:0046872 IEA
    triokinase activity GO:0050354 IDA
    triokinase activity GO:0050354 IEA
    triokinase activity GO:0050354 ISO
Biological Process
    carbohydrate metabolic process GO:0005975 ISO
    glycerol metabolic process GO:0006071 IEA
    glycerol catabolic process GO:0019563 IBA
    negative regulation of MDA-5 signaling pathway GO:0039534 IEA
    negative regulation of MDA-5 signaling pathway GO:0039534 ISO
    negative regulation of MDA-5 signaling pathway GO:0039534 ISS
    regulation of innate immune response GO:0045088 ISO
    carbohydrate phosphorylation GO:0046835 IEA
    carbohydrate phosphorylation GO:0046835 ISO
    fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate GO:0061624 IEA
    fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate GO:0061624 ISO
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified Tkfc in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tkfc
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tkfc is involved
PathwayEvidenceSource
Fructose catabolism IEA Reactome
Fructose metabolism IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome





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