Gene description for Ubac1
Gene name UBA domain containing 1
Gene symbol Ubac1
Other names/aliases Ubadc1
Species Rattus norvegicus
 Database cross references - Ubac1
ExoCarta ExoCarta_362087
Vesiclepedia VP_362087
Entrez Gene 362087
UniProt Q5XIR9  
 Ubac1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ubac1
Molecular Function
    polyubiquitin modification-dependent protein binding GO:0031593 IEA
    polyubiquitin modification-dependent protein binding GO:0031593 ISO
    polyubiquitin modification-dependent protein binding GO:0031593 ISS
    proteasome binding GO:0070628 IEA
    proteasome binding GO:0070628 ISO
    proteasome binding GO:0070628 ISS
Biological Process
    protein ubiquitination GO:0016567 IEA
    protein ubiquitination GO:0016567 ISO
    protein ubiquitination GO:0016567 ISS
    protein maturation GO:0051604 IEA
    protein maturation GO:0051604 ISO
    protein maturation GO:0051604 ISS
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IBA
    cytoplasm GO:0005737 IEA
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Ubac1 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 101
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ubac1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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