Gene description for Pck1
Gene name phosphoenolpyruvate carboxykinase 1 (soluble)
Gene symbol Pck1
Other names/aliases GTP
PCK
PEPCK-C
Pepck
RATPEPCK
Species Rattus norvegicus
 Database cross references - Pck1
ExoCarta ExoCarta_362282
Entrez Gene 362282
UniProt P07379  
 Pck1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Pck1
Molecular Function
    GTP binding GO:0005525 ISO
    GDP binding GO:0019003 IDA
    phosphoenolpyruvate carboxykinase (GTP) activity GO:0004613 IDA
    magnesium ion binding GO:0000287 ISS
    manganese ion binding GO:0030145 ISO
    carboxylic acid binding GO:0031406 ISO
    phosphoenolpyruvate carboxykinase activity GO:0004611 ISO
Biological Process
    positive regulation of transcription from RNA polymerase II promoter in response to acidic pH GO:0061402 ISO
    gluconeogenesis GO:0006094 TAS
    glucose homeostasis GO:0042593 IDA
    response to activity GO:0014823 IEP
    lipid metabolic process GO:0006629 ISO
    glycerol biosynthetic process from pyruvate GO:0046327 ISO
    cellular response to potassium ion starvation GO:0051365 ISO
    oxaloacetate metabolic process GO:0006107 IDA
    response to insulin GO:0032868 ISS
    internal protein amino acid acetylation GO:0006475 ISS
    glucose metabolic process GO:0006006 ISO
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Pck1 in exosomes
1
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Pck1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pck1 is involved
PathwayEvidenceSource
Abacavir metabolism IEA Reactome
Gluconeogenesis IEA Reactome





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