Gene description for Smpdl3b
Gene name sphingomyelin phosphodiesterase, acid-like 3B
Gene symbol Smpdl3b
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Smpdl3b
ExoCarta ExoCarta_362619
Vesiclepedia VP_362619
Entrez Gene 362619
 Smpdl3b identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Smpdl3b
Molecular Function
    sphingomyelin phosphodiesterase activity GO:0004767 IEA
    phosphoric diester hydrolase activity GO:0008081 IBA
    phosphoric diester hydrolase activity GO:0008081 ISO
    zinc ion binding GO:0008270 ISO
Biological Process
    sphingomyelin catabolic process GO:0006685 IEA
    negative regulation of toll-like receptor signaling pathway GO:0034122 ISO
    membrane lipid catabolic process GO:0046466 ISO
    negative regulation of inflammatory response GO:0050728 ISO
Subcellular Localization
    extracellular space GO:0005615 IBA
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Smpdl3b in sEVs
1
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Smpdl3b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Smpdl3b is involved
No pathways found





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