ExoCarta: Gene summary

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Gene description for Fam213b
Gene name	family with sequence similarity 213, member B
Gene symbol	Fam213b
Other names/aliases	RGD1308251
Species	Rattus norvegicus
Database cross references - Fam213b
ExoCarta	ExoCarta_362676
Entrez Gene	362676
UniProt	D3ZVR7
Fam213b identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells	20124479
Pancreatic cancer cells	20124479

Gene ontology annotations for Fam213b

Molecular Function
	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	GO:0016616	ISS
	prostaglandin-F synthase activity	GO:0047017	ISS
Biological Process
	prostaglandin biosynthetic process	GO:0001516	ISS
	oxidation-reduction process	GO:0055114	ISO
Subcellular Localization
	myelin sheath	GO:0043209	ISO
	cytoplasm	GO:0005737	ISS
	endoplasmic reticulum	GO:0005783	ISS
	cytosol	GO:0005829	ISO
	extracellular exosome	GO:0070062	ISO

Experiment description of studies that identified Fam213b in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ HSP70\|GAPDH	EV Cytosolic markers
✔ CD9\|CD151	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

mrna

Identification method

Microarray

PubMed ID

20124479

Organism

Rattus norvegicus

Experiment description

Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.

Authors

Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.

Journal name

Publication year

2010

Sample

Pancreatic cancer cells

Sample name

Isolation/purification methods

Differential centrifugation
Sucrose density gradient

Flotation density

1.14-1.17 g/mL

Molecules identified in the study

Protein
mRNA

Methods used in the study

RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]