Gene description for Hectd1
Gene name HECT domain containing E3 ubiquitin protein ligase 1
Gene symbol Hectd1
Other names/aliases RGD1561653
Species Rattus norvegicus
 Database cross references - Hectd1
ExoCarta ExoCarta_362736
Entrez Gene 362736
 Hectd1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Hectd1
Molecular Function
    metal ion binding GO:0046872 IEA
    ligase activity GO:0016874 IEA
    ubiquitin-protein transferase activity GO:0004842 IBA
    ubiquitin protein ligase activity GO:0061630 ISO
Biological Process
    embryonic placenta development GO:0001892 ISO
    natural killer cell differentiation GO:0001779 ISO
    protein K63-linked ubiquitination GO:0070534 ISO
    negative regulation of protein localization to plasma membrane GO:1903077 ISO
    neural tube closure GO:0001843 ISO
    protein autoubiquitination GO:0051865 ISO
    spongiotrophoblast differentiation GO:0060708 ISO
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IBA
    trophoblast giant cell differentiation GO:0060707 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified Hectd1 in exosomes
1
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hectd1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hectd1 is involved
No pathways found





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