Gene description for Hectd1
Gene name HECT domain containing E3 ubiquitin protein ligase 1
Gene symbol Hectd1
Other names/aliases RGD1561653
Species Rattus norvegicus
 Database cross references - Hectd1
ExoCarta ExoCarta_362736
Vesiclepedia VP_362736
Entrez Gene 362736
 Hectd1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Hectd1
Molecular Function
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 ISO
Biological Process
    protein polyubiquitination GO:0000209 IBA
    natural killer cell differentiation GO:0001779 ISO
    neural tube closure GO:0001843 ISO
    neural tube closure GO:0001843 ISO
    embryonic placenta development GO:0001892 ISO
    heart valve development GO:0003170 ISO
    ventricular septum development GO:0003281 ISO
    aorta development GO:0035904 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    protein autoubiquitination GO:0051865 ISO
    trophoblast giant cell differentiation GO:0060707 ISO
    spongiotrophoblast differentiation GO:0060708 ISO
    protein K63-linked ubiquitination GO:0070534 ISO
    protein localization to plasma membrane GO:0072659 ISO
    negative regulation of protein localization to plasma membrane GO:1903077 ISO
Subcellular Localization
    nuclear speck GO:0016607 IBA
 Experiment description of studies that identified Hectd1 in sEVs
1
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hectd1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hectd1 is involved
No pathways found





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