Gene description for Ptges3
Gene name prostaglandin E synthase 3 (cytosolic)
Gene symbol Ptges3
Other names/aliases RGD1561913
Species Rattus norvegicus
 Database cross references - Ptges3
ExoCarta ExoCarta_362809
Vesiclepedia VP_362809
Entrez Gene 362809
UniProt P83868  
 Ptges3 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ptges3
Molecular Function
    p53 binding GO:0002039 IDA
    telomerase activity GO:0003720 IEA
    telomerase activity GO:0003720 ISO
    isomerase activity GO:0016853 IEA
    enzyme binding GO:0019899 IPI
    prostaglandin-E synthase activity GO:0050220 IBA
    prostaglandin-E synthase activity GO:0050220 IDA
    prostaglandin-E synthase activity GO:0050220 IEA
    prostaglandin-E synthase activity GO:0050220 ISO
    prostaglandin-E synthase activity GO:0050220 ISS
    unfolded protein binding GO:0051082 IEA
    unfolded protein binding GO:0051082 ISO
    unfolded protein binding GO:0051082 ISS
    protein-folding chaperone binding GO:0051087 IBA
    protein-folding chaperone binding GO:0051087 IEA
    Hsp90 protein binding GO:0051879 IBA
    Hsp90 protein binding GO:0051879 IDA
    Hsp90 protein binding GO:0051879 IEA
    Hsp90 protein binding GO:0051879 ISO
    DNA polymerase binding GO:0070182 IEA
    DNA polymerase binding GO:0070182 ISO
Biological Process
    prostaglandin biosynthetic process GO:0001516 IBA
    prostaglandin biosynthetic process GO:0001516 IDA
    prostaglandin biosynthetic process GO:0001516 IEA
    prostaglandin biosynthetic process GO:0001516 IMP
    prostaglandin biosynthetic process GO:0001516 ISO
    prostaglandin biosynthetic process GO:0001516 ISO
    prostaglandin biosynthetic process GO:0001516 ISS
    glycogen biosynthetic process GO:0005978 IEA
    glycogen biosynthetic process GO:0005978 ISO
    protein folding GO:0006457 IBA
    prostaglandin metabolic process GO:0006693 IMP
    telomere maintenance via telomerase GO:0007004 IBA
    telomere maintenance via telomerase GO:0007004 IEA
    telomere maintenance via telomerase GO:0007004 ISO
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of telomere maintenance via telomerase GO:0032212 IEA
    positive regulation of telomere maintenance via telomerase GO:0032212 ISO
    nuclear receptor-mediated glucocorticoid signaling pathway GO:0042921 IEA
    nuclear receptor-mediated glucocorticoid signaling pathway GO:0042921 ISO
    skin development GO:0043588 IEA
    skin development GO:0043588 ISO
    fibroblast proliferation GO:0048144 IEA
    fibroblast proliferation GO:0048144 ISO
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    chaperone cofactor-dependent protein refolding GO:0051085 IEA
    chaperone cofactor-dependent protein refolding GO:0051085 ISO
    chaperone-mediated protein complex assembly GO:0051131 IBA
    chaperone-mediated protein complex assembly GO:0051131 IEA
    chaperone-mediated protein complex assembly GO:0051131 ISO
    neuron apoptotic process GO:0051402 IMP
    lung saccule development GO:0060430 IEA
    lung saccule development GO:0060430 ISO
    telomerase holoenzyme complex assembly GO:1905323 IBA
    telomerase holoenzyme complex assembly GO:1905323 IEA
    telomerase holoenzyme complex assembly GO:1905323 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    telomerase holoenzyme complex GO:0005697 IEA
    telomerase holoenzyme complex GO:0005697 ISO
    cytoplasm GO:0005737 IEA
    cytosol GO:0005829 IBA
    actin filament GO:0005884 IDA
    protein-containing complex GO:0032991 IDA
    protein-containing complex GO:0032991 ISO
    neuronal cell body GO:0043025 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    protein folding chaperone complex GO:0101031 IEA
    protein folding chaperone complex GO:0101031 ISO
 Experiment description of studies that identified Ptges3 in sEVs
1
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 101
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ptges3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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