Gene description for Sppl2b
Gene name signal peptide peptidase-like 2B
Gene symbol Sppl2b
Other names/aliases RGD1308556
Species Rattus norvegicus
 Database cross references - Sppl2b
ExoCarta ExoCarta_362828
Entrez Gene 362828
UniProt Q5PQL3  
 Sppl2b identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Sppl2b
Molecular Function
    aspartic endopeptidase activity, intramembrane cleaving GO:0042500 ISS
    protein homodimerization activity GO:0042803 ISS
Biological Process
    membrane protein ectodomain proteolysis GO:0006509 ISS
    regulation of immune response GO:0050776 ISS
    membrane protein intracellular domain proteolysis GO:0031293 ISS
Subcellular Localization
    plasma membrane GO:0005886 ISS
    integral component of cytoplasmic side of endoplasmic reticulum membrane GO:0071458 ISS
    integral component of lumenal side of endoplasmic reticulum membrane GO:0071556 ISS
    Golgi-associated vesicle membrane GO:0030660 ISS
    endosome membrane GO:0010008 ISS
    lysosomal membrane GO:0005765 ISS
 Experiment description of studies that identified Sppl2b in exosomes
1
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Sppl2b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Sppl2b is involved
No pathways found





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