Gene description for Wdr61
Gene name WD repeat domain 61
Gene symbol Wdr61
Other names/aliases RGD1308228
Species Rattus norvegicus
 Database cross references - Wdr61
ExoCarta ExoCarta_363064
Vesiclepedia VP_363064
Entrez Gene 363064
UniProt Q4V7A0  
 Wdr61 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Wdr61
Biological Process
    transcription elongation by RNA polymerase II GO:0006368 IEA
    transcription elongation by RNA polymerase II GO:0006368 ISO
    transcription elongation by RNA polymerase II GO:0006368 ISS
    Wnt signaling pathway GO:0016055 IEA
    negative regulation of myeloid cell differentiation GO:0045638 IEA
    negative regulation of myeloid cell differentiation GO:0045638 ISO
    negative regulation of myeloid cell differentiation GO:0045638 ISS
    nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay GO:0070478 IEA
    nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay GO:0070478 ISO
    nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay GO:0070478 ISS
    rescue of stalled ribosome GO:0072344 IEA
    rescue of stalled ribosome GO:0072344 ISO
    rescue of stalled ribosome GO:0072344 ISS
Subcellular Localization
    euchromatin GO:0000791 IEA
    euchromatin GO:0000791 ISO
    euchromatin GO:0000791 ISS
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    Cdc73/Paf1 complex GO:0016593 IBA
    Cdc73/Paf1 complex GO:0016593 IEA
    Cdc73/Paf1 complex GO:0016593 ISO
    Cdc73/Paf1 complex GO:0016593 ISS
    Ski complex GO:0055087 IEA
    Ski complex GO:0055087 ISO
    Ski complex GO:0055087 ISS
 Experiment description of studies that identified Wdr61 in sEVs
1
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Wdr61
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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