Gene description for Gmppb
Gene name GDP-mannose pyrophosphorylase B
Gene symbol Gmppb
Other names/aliases RGD1560458
Species Rattus norvegicus
 Database cross references - Gmppb
ExoCarta ExoCarta_363145
Vesiclepedia VP_363145
Entrez Gene 363145
 Gmppb identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Gmppb
Molecular Function
    mannose-1-phosphate guanylyltransferase (GTP) activity GO:0004475 IBA
    mannose-1-phosphate guanylyltransferase (GTP) activity GO:0004475 ISO
    GTP binding GO:0005525 IEA
Biological Process
    protein glycosylation GO:0006486 IBA
    GDP-mannose biosynthetic process GO:0009298 IBA
    GDP-mannose biosynthetic process GO:0009298 ISO
    GDP-mannose metabolic process GO:0019673 ISO
    GDP-mannose biosynthetic process from mannose GO:0061728 ISO
    GDP-mannose biosynthetic process from glucose GO:0141199 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    GDP-mannose pyrophosphorylase complex GO:0120508 ISO
 Experiment description of studies that identified Gmppb in sEVs
1
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Gmppb
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gmppb is involved
No pathways found





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