Gene description for Atg9a
Gene name autophagy related 9A
Gene symbol Atg9a
Other names/aliases RGD1310450
Species Rattus norvegicus
 Database cross references - Atg9a
ExoCarta ExoCarta_363254
Vesiclepedia VP_363254
Entrez Gene 363254
UniProt Q5FWU3  
 Atg9a identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Atg9a
Molecular Function
    phospholipid scramblase activity GO:0017128 IEA
    phospholipid scramblase activity GO:0017128 ISO
    phospholipid scramblase activity GO:0017128 ISS
Biological Process
    autophagosome assembly GO:0000045 IEA
    autophagosome assembly GO:0000045 ISO
    autophagosome assembly GO:0000045 ISO
    autophagosome assembly GO:0000045 ISS
    mitophagy GO:0000423 IBA
    autophagy GO:0006914 ISO
    autophagy GO:0006914 ISO
    negative regulation of macrophage cytokine production GO:0010936 ISO
    plasma membrane phospholipid scrambling GO:0017121 IEA
    response to nutrient levels GO:0031667 IEP
    negative regulation of interferon-beta production GO:0032688 ISO
    protein localization to Golgi apparatus GO:0034067 ISO
    protein localization to phagophore assembly site GO:0034497 IBA
    piecemeal microautophagy of the nucleus GO:0034727 IBA
    innate immune response GO:0045087 ISO
    bone morphogenesis GO:0060349 ISO
    bone morphogenesis GO:0060349 ISS
    reticulophagy GO:0061709 IBA
    programmed necrotic cell death GO:0097300 ISO
    programmed necrotic cell death GO:0097300 ISS
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi membrane GO:0000139 ISO
    Golgi membrane GO:0000139 ISS
    phagophore assembly site GO:0000407 IBA
    phagophore assembly site GO:0000407 ISO
    autophagosome membrane GO:0000421 IEA
    autophagosome membrane GO:0000421 ISO
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    endosome GO:0005768 ISO
    late endosome GO:0005770 IDA
    late endosome GO:0005770 ISO
    autophagosome GO:0005776 IBA
    autophagosome GO:0005776 ISO
    endoplasmic reticulum membrane GO:0005789 IEA
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISS
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    trans-Golgi network GO:0005802 IEA
    trans-Golgi network GO:0005802 ISO
    trans-Golgi network GO:0005802 ISS
    synaptic vesicle GO:0008021 EXP
    synaptic vesicle GO:0008021 IDA
    late endosome membrane GO:0031902 IEA
    mitochondrial membrane GO:0031966 IEA
    phagophore assembly site membrane GO:0034045 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
    recycling endosome GO:0055037 ISO
    recycling endosome membrane GO:0055038 IEA
    recycling endosome membrane GO:0055038 ISO
    recycling endosome membrane GO:0055038 ISS
    synaptic membrane GO:0097060 ISO
 Experiment description of studies that identified Atg9a in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Atg9a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Atg9a is involved
PathwayEvidenceSource
Autophagy IEA Reactome
Macroautophagy IEA Reactome
Mitophagy IEA Reactome
PINK1-PRKN Mediated Mitophagy IEA Reactome
Selective autophagy IEA Reactome





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