Gene description for Obsl1
Gene name obscurin-like 1
Gene symbol Obsl1
Other names/aliases RGD1306073
Species Rattus norvegicus
 Database cross references - Obsl1
ExoCarta ExoCarta_363259
Entrez Gene 363259
UniProt D3ZZ80  
 Obsl1 identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Obsl1
Biological Process
    regulation of mitotic nuclear division GO:0007088 ISS
    positive regulation of dendrite morphogenesis GO:0050775 ISO
    protein localization to Golgi apparatus GO:0034067 ISO
    microtubule cytoskeleton organization GO:0000226 ISS
    Golgi organization GO:0007030 ISO
Subcellular Localization
    Golgi apparatus GO:0005794 ISS
    perinuclear region of cytoplasm GO:0048471 ISS
    3M complex GO:1990393 ISS
    centrosome GO:0005813 ISS
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified Obsl1 in exosomes
1
Experiment ID 92
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Obsl1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Obsl1 is involved
No pathways found





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