Gene description for Agfg1
Gene name ArfGAP with FG repeats 1
Gene symbol Agfg1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Agfg1
ExoCarta ExoCarta_363266
Entrez Gene 363266
UniProt Q4KLH5  
 Agfg1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Agfg1
Molecular Function
    DNA binding GO:0003677 IEA
    metal ion binding GO:0046872 IEA
    GTPase activator activity GO:0005096 IEA
Biological Process
    positive regulation of GTPase activity GO:0043547 IEA
    multicellular organismal development GO:0007275 IEA
    acrosome assembly GO:0001675 ISO
    spermatid nucleus differentiation GO:0007289 ISO
    intermediate filament organization GO:0045109 ISO
    transport GO:0006810 IEA
Subcellular Localization
    cytoplasmic membrane-bounded vesicle GO:0016023 ISO
    neuronal cell body GO:0043025 ISO
    cell projection GO:0042995 ISO
    nucleus GO:0005634 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Agfg1 in exosomes
1
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Agfg1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Agfg1 is involved
No pathways found





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