Gene description for Usp9x
Gene name ubiquitin specific peptidase 9, X-linked
Gene symbol Usp9x
Other names/aliases RGD1560056
Species Rattus norvegicus
 Database cross references - Usp9x
ExoCarta ExoCarta_363445
Entrez Gene 363445
 Usp9x identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Usp9x
Molecular Function
    ubiquitin-specific protease activity GO:0004843 IBA
    cysteine-type endopeptidase activity GO:0004197 IBA
    co-SMAD binding GO:0070410 ISO
Biological Process
    cerebellar cortex structural organization GO:0021698 ISO
    hippocampus development GO:0021766 ISO
    protein deubiquitination GO:0016579 ISO
    cellular response to transforming growth factor beta stimulus GO:0071560 ISO
    BMP signaling pathway GO:0030509 ISO
    neuron projection extension GO:1990138 ISO
    regulation of proteasomal protein catabolic process GO:0061136 IBA
    neuron migration GO:0001764 ISO
    axon extension GO:0048675 ISO
    post-embryonic development GO:0009791 ISO
    in utero embryonic development GO:0001701 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    transforming growth factor beta receptor signaling pathway GO:0007179 ISO
    cell migration GO:0016477 IBA
Subcellular Localization
    membrane GO:0016020 ISO
    growth cone GO:0030426 ISO
    apical part of cell GO:0045177 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Usp9x in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Usp9x
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Usp9x is involved
No pathways found





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