Gene description for Tmem165
Gene name transmembrane protein 165
Gene symbol Tmem165
Other names/aliases Tparl
Species Rattus norvegicus
 Database cross references - Tmem165
ExoCarta ExoCarta_364137
Entrez Gene 364137
UniProt Q4V899  
 Tmem165 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Tmem165
Biological Process
    protein N-linked glycosylation GO:0006487 ISS
    regulation of lysosomal lumen pH GO:0035751 ISS
    cellular calcium ion homeostasis GO:0006874 ISS
    Golgi calcium ion transport GO:0032472 ISS
Subcellular Localization
    endosome membrane GO:0010008 ISS
    late endosome membrane GO:0031902 IEA
    trans-Golgi network membrane GO:0032588 ISS
    Golgi apparatus GO:0005794 ISS
    early endosome membrane GO:0031901 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
    lysosomal membrane GO:0005765 ISS
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified Tmem165 in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tmem165
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tmem165 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here