Gene description for Plek
Gene name pleckstrin
Gene symbol Plek
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Plek
ExoCarta ExoCarta_364206
Vesiclepedia VP_364206
Entrez Gene 364206
UniProt Q4KM33  
 Plek identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Plek
Molecular Function
    protein kinase C binding GO:0005080 ISO
    protein homodimerization activity GO:0042803 ISO
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 ISO
Biological Process
    hematopoietic progenitor cell differentiation GO:0002244 ISO
    platelet degranulation GO:0002576 ISO
    vesicle docking involved in exocytosis GO:0006904 IEA
    vesicle docking involved in exocytosis GO:0006904 ISO
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 IEA
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 ISO
    integrin-mediated signaling pathway GO:0007229 ISO
    positive regulation of platelet activation GO:0010572 IEA
    positive regulation of platelet activation GO:0010572 ISO
    negative regulation of inositol phosphate biosynthetic process GO:0010920 ISO
    positive regulation of inositol-polyphosphate 5-phosphatase activity GO:0010925 ISO
    cell projection organization GO:0030030 ISO
    actin cytoskeleton organization GO:0030036 IBA
    actin cytoskeleton organization GO:0030036 ISO
    positive regulation of actin filament depolymerization GO:0030836 IEA
    positive regulation of actin filament depolymerization GO:0030836 ISO
    phospholipase C-inhibiting G protein-coupled receptor signaling pathway GO:0030845 ISO
    cortical actin cytoskeleton organization GO:0030866 ISO
    ruffle organization GO:0031529 ISO
    positive regulation of actin filament bundle assembly GO:0032233 ISO
    positive regulation of integrin activation GO:0033625 IEA
    positive regulation of integrin activation GO:0033625 ISO
    intracellular signal transduction GO:0035556 IEA
    negative regulation of G protein-coupled receptor signaling pathway GO:0045744 ISO
    phosphatidylinositol metabolic process GO:0046488 ISO
    regulation of cell diameter GO:0060305 ISO
    thrombin-activated receptor signaling pathway GO:0070493 IEA
    thrombin-activated receptor signaling pathway GO:0070493 ISO
    platelet aggregation GO:0070527 IEA
    platelet aggregation GO:0070527 ISO
    protein secretion by platelet GO:0070560 IEA
    protein secretion by platelet GO:0070560 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 IBA
    ruffle membrane GO:0032587 ISO
 Experiment description of studies that identified Plek in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Plek
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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