Gene description for Tmem55b
Gene name transmembrane protein 55B
Gene symbol Tmem55b
Other names/aliases RGD1307475
Species Rattus norvegicus
 Database cross references - Tmem55b
ExoCarta ExoCarta_364298
Vesiclepedia VP_364298
Entrez Gene 364298
UniProt Q5PPM8  
 Tmem55b identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Tmem55b
Molecular Function
    phosphatidylinositol-4,5-bisphosphate 4-phosphatase activity GO:0034597 IBA
    phosphatidylinositol-4,5-bisphosphate 4-phosphatase activity GO:0034597 IEA
    phosphatidylinositol-4,5-bisphosphate 4-phosphatase activity GO:0034597 ISO
Biological Process
    response to sterol depletion GO:0006991 ISO
    response to sterol depletion GO:0006991 ISS
    cholesterol metabolic process GO:0008203 ISO
    cholesterol metabolic process GO:0008203 ISS
    lysosome localization GO:0032418 ISO
    lysosome localization GO:0032418 ISS
    phosphatidylinositol dephosphorylation GO:0046856 IBA
    phosphatidylinositol dephosphorylation GO:0046856 IEA
    phosphatidylinositol dephosphorylation GO:0046856 ISO
    proton-transporting V-type ATPase complex assembly GO:0070070 ISO
    proton-transporting V-type ATPase complex assembly GO:0070070 ISS
    positive regulation of TORC1 signaling GO:1904263 ISO
    positive regulation of TORC1 signaling GO:1904263 ISS
Subcellular Localization
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IEA
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    phagocytic vesicle membrane GO:0030670 IBA
    phagocytic vesicle membrane GO:0030670 IEA
    phagocytic vesicle membrane GO:0030670 ISO
    phagocytic vesicle membrane GO:0030670 ISS
    late endosome membrane GO:0031902 IBA
    late endosome membrane GO:0031902 IEA
    late endosome membrane GO:0031902 ISO
    late endosome membrane GO:0031902 ISS
 Experiment description of studies that identified Tmem55b in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tmem55b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here