ExoCarta: Gene summary

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Gene description for Prmt5
Gene name	protein arginine methyltransferase 5
Gene symbol	Prmt5
Other names/aliases	Skb1
Species	Rattus norvegicus
Database cross references - Prmt5
ExoCarta	ExoCarta_364382
Entrez Gene	364382
Prmt5 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Prmt5

Molecular Function
	protein complex binding	GO:0032403	IDA
	methyltransferase activity	GO:0008168	ISO
	core promoter sequence-specific DNA binding	GO:0001046	ISO
	ribonucleoprotein complex binding	GO:0043021	ISO
	transcription corepressor activity	GO:0003714	ISO
	chromatin binding	GO:0003682	ISO
	histone-arginine N-methyltransferase activity	GO:0008469	IBA
	protein-arginine omega-N symmetric methyltransferase activity	GO:0035243	ISO
	protein heterodimerization activity	GO:0046982	ISO
Biological Process
	protein methylation	GO:0006479	IMP
	regulation of ERK1 and ERK2 cascade	GO:0070372	IMP
	peptidyl-arginine methylation	GO:0018216	ISO
	regulation of transcription, DNA-templated	GO:0006355	IBA
	circadian regulation of gene expression	GO:0032922	ISO
	regulation of DNA methylation	GO:0044030	IMP
	peptidyl-arginine N-methylation	GO:0035246	ISO
	histone H4-R3 methylation	GO:0043985	ISO
	histone arginine methylation	GO:0034969	IBA
	endothelial cell activation	GO:0042118	ISO
	liver regeneration	GO:0097421	IEP
	negative regulation of cell differentiation	GO:0045596	IMP
	negative regulation of transcription from RNA polymerase II promoter	GO:0000122	ISO
	peptidyl-arginine methylation, to symmetrical-dimethyl arginine	GO:0019918	ISO
	spliceosomal snRNP assembly	GO:0000387	ISO
	positive regulation of oligodendrocyte differentiation	GO:0048714	IMP
Subcellular Localization
	methylosome	GO:0034709	ISO
	nucleus	GO:0005634	ISO
	cytoplasm	GO:0005737	ISO
	cytosol	GO:0005829	ISO
	protein complex	GO:0043234	IDA

Experiment description of studies that identified Prmt5 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D4 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting