Gene description for Prmt5
Gene name protein arginine methyltransferase 5
Gene symbol Prmt5
Other names/aliases Skb1
Species Rattus norvegicus
 Database cross references - Prmt5
ExoCarta ExoCarta_364382
Vesiclepedia VP_364382
Entrez Gene 364382
 Prmt5 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Prmt5
Molecular Function
    p53 binding GO:0002039 ISO
    chromatin binding GO:0003682 ISO
    transcription corepressor activity GO:0003714 ISO
    methyltransferase activity GO:0008168 ISO
    methyl-CpG binding GO:0008327 ISO
    histone arginine N-methyltransferase activity GO:0008469 IBA
    protein-arginine N-methyltransferase activity GO:0016274 ISO
    protein-arginine omega-N symmetric methyltransferase activity GO:0035243 ISO
    histone H3R17 methyltransferase activity GO:0035642 IEA
    identical protein binding GO:0042802 ISO
    ribonucleoprotein complex binding GO:0043021 ISO
    histone H4R3 methyltransferase activity GO:0044020 ISO
    protein-containing complex binding GO:0044877 IDA
    protein heterodimerization activity GO:0046982 ISO
    histone H3K37 methyltransferase activity GO:0062122 IEA
    histone H3R2 methyltransferase activity GO:0070611 IEA
    histone H2AR3 methyltransferase activity GO:0070612 ISO
    E-box binding GO:0070888 ISO
    histone H3R8 methyltransferase activity GO:0140592 IEA
    histone H3K56 methyltransferase activity GO:0140759 IEA
    histone H3R26 methyltransferase activity GO:0140903 IEA
    histone H4K12 methyltransferase activity GO:0140984 IEA
    histone H2AQ104 methyltransferase activity GO:1990259 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    spliceosomal snRNP assembly GO:0000387 ISO
    chromatin remodeling GO:0006338 ISO
    DNA-templated transcription termination GO:0006353 ISO
    regulation of DNA-templated transcription GO:0006355 IBA
    protein methylation GO:0006479 IMP
    gene expression GO:0010467 ISO
    regulation of gene expression GO:0010468 IMP
    peptidyl-arginine methylation GO:0018216 ISO
    methylation GO:0032259 IEA
    circadian regulation of gene expression GO:0032922 ISO
    peptidyl-arginine N-methylation GO:0035246 ISO
    endothelial cell activation GO:0042118 ISO
    negative regulation of gene expression via chromosomal CpG island methylation GO:0044027 IMP
    negative regulation of cell differentiation GO:0045596 IMP
    positive regulation of oligodendrocyte differentiation GO:0048714 IMP
    regulation of ERK1 and ERK2 cascade GO:0070372 IMP
    Golgi ribbon formation GO:0090161 ISO
    liver regeneration GO:0097421 IEP
    positive regulation of adenylate cyclase-inhibiting dopamine receptor signaling pathway GO:1904992 ISO
Subcellular Localization
    chromatin GO:0000785 ISO
    male germ cell nucleus GO:0001673 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    protein-containing complex GO:0032991 IDA
    methylosome GO:0034709 ISO
    histone methyltransferase complex GO:0035097 ISO
 Experiment description of studies that identified Prmt5 in sEVs
1
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Prmt5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Prmt5 is involved
No pathways found





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