Gene description for Chmp2a
Gene name charged multivesicular body protein 2A
Gene symbol Chmp2a
Other names/aliases RGD1305050
Species Rattus norvegicus
 Database cross references - Chmp2a
ExoCarta ExoCarta_365191
Vesiclepedia VP_365191
Entrez Gene 365191
 Chmp2a identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Chmp2a
Molecular Function
    protein domain specific binding GO:0019904 ISO
    phosphatidylcholine binding GO:0031210 ISO
Biological Process
    plasma membrane repair GO:0001778 ISO
    autophagy GO:0006914 ISO
    nucleus organization GO:0006997 ISO
    mitotic metaphase chromosome alignment GO:0007080 ISO
    membrane invagination GO:0010324 ISO
    exit from mitosis GO:0010458 ISO
    regulation of centrosome duplication GO:0010824 ISO
    nuclear membrane reassembly GO:0031468 ISO
    viral budding via host ESCRT complex GO:0039702 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    establishment of protein localization GO:0045184 ISO
    viral budding from plasma membrane GO:0046761 ISO
    protein polymerization GO:0051258 ISO
    protein homooligomerization GO:0051260 ISO
    midbody abscission GO:0061952 ISO
    multivesicular body sorting pathway GO:0071985 ISO
    autophagosome maturation GO:0097352 ISO
    regulation of mitotic spindle assembly GO:1901673 ISO
    late endosome to lysosome transport GO:1902774 ISO
    positive regulation of exosomal secretion GO:1903543 ISO
    negative regulation of centriole elongation GO:1903723 ISO
Subcellular Localization
    autophagosome membrane GO:0000421 ISO
    kinetochore GO:0000776 ISO
    chromatin GO:0000785 ISO
    ESCRT III complex GO:0000815 ISO
    nuclear envelope GO:0005635 ISO
    nuclear pore GO:0005643 ISO
    lysosomal membrane GO:0005765 ISO
    kinetochore microtubule GO:0005828 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    membrane coat GO:0030117 ISO
    midbody GO:0030496 ISO
    multivesicular body membrane GO:0032585 ISO
    amphisome membrane GO:1904930 ISO
 Experiment description of studies that identified Chmp2a in sEVs
1
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Chmp2a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Chmp2a is involved
No pathways found





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