Gene description for Nadk2
Gene name NAD kinase 2, mitochondrial
Gene symbol Nadk2
Other names/aliases Nadkd1
RGD1306809
Species Rattus norvegicus
 Database cross references - Nadk2
ExoCarta ExoCarta_365699
Vesiclepedia VP_365699
Entrez Gene 365699
UniProt Q1HCL7  
 Nadk2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Nadk2
Molecular Function
    NAD+ kinase activity GO:0003951 IBA
    NAD+ kinase activity GO:0003951 IEA
    NAD+ kinase activity GO:0003951 ISO
    NAD+ kinase activity GO:0003951 ISS
    ATP binding GO:0005524 IEA
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
Biological Process
    NADP biosynthetic process GO:0006741 IEA
    biological_process GO:0008150 ND
    NAD metabolic process GO:0019674 IBA
    NAD metabolic process GO:0019674 IEA
    NAD metabolic process GO:0019674 ISO
    NAD metabolic process GO:0019674 ISS
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
 Experiment description of studies that identified Nadk2 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Nadk2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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