Gene description for Bag5
Gene name BCL2-associated athanogene 5
Gene symbol Bag5
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Bag5
ExoCarta ExoCarta_366734
Vesiclepedia VP_366734
Entrez Gene 366734
UniProt Q5QJC9  
 Bag5 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Bag5
Molecular Function
    adenyl-nucleotide exchange factor activity GO:0000774 IBA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 ISO
    ubiquitin protein ligase binding GO:0031625 IPI
    ubiquitin protein ligase binding GO:0031625 ISO
    protein-folding chaperone binding GO:0051087 IBA
    protein-folding chaperone binding GO:0051087 IDA
    protein-folding chaperone binding GO:0051087 ISO
Biological Process
    Golgi organization GO:0007030 ISO
    negative regulation of neuron projection development GO:0010977 ISO
    negative regulation of protein ubiquitination GO:0031397 IBA
    negative regulation of protein ubiquitination GO:0031397 IDA
    negative regulation of protein ubiquitination GO:0031397 ISO
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 ISO
    protein stabilization GO:0050821 IBA
    protein stabilization GO:0050821 ISO
    negative regulation of ubiquitin-protein transferase activity GO:0051444 IDA
    negative regulation of protein refolding GO:0061084 IMP
    regulation of inclusion body assembly GO:0090083 IBA
    regulation of inclusion body assembly GO:0090083 IDA
    regulation of inclusion body assembly GO:0090083 ISO
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    membrane GO:0016020 IBA
    inclusion body GO:0016234 ISO
    inclusion body GO:0016234 ISS
    junctional membrane complex GO:0030314 ISO
    junctional membrane complex GO:0030314 ISS
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
 Experiment description of studies that identified Bag5 in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Bag5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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