Gene description for Zasp52
Gene name Z band alternatively spliced PDZ-motif protein 52
Gene symbol Zasp52
Other names/aliases CG12968
CG12969
CG30084
CG8242
DmelCG30084
Z(210)
ZASP52
Zasp
Zbp210
dzasp
tun
tungus
zasp
zasp52
Species Drosophila melanogaster
 Database cross references - Zasp52
ExoCarta ExoCarta_36740
Vesiclepedia VP_36740
Entrez Gene 36740
UniProt A1ZA47  
 Zasp52 identified in sEVs derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Zasp52
Molecular Function
    actin binding GO:0003779 IPI
    actinin binding GO:0042805 IPI
    muscle alpha-actinin binding GO:0051371 IMP
    muscle alpha-actinin binding GO:0051371 IPI
Biological Process
    regulation of cell-matrix adhesion GO:0001952 IMP
    myofibril assembly GO:0030239 IMP
    muscle structure development GO:0061061 IMP
Subcellular Localization
    stress fiber GO:0001725 IDA
    zonula adherens GO:0005915 IDA
    focal adhesion GO:0005925 IDA
    muscle tendon junction GO:0005927 IDA
    actin cytoskeleton GO:0015629 IDA
    basolateral plasma membrane GO:0016323 IDA
    Z disc GO:0030018 IDA
    cell leading edge GO:0031252 IDA
    apical part of cell GO:0045177 IDA
    basal part of cell GO:0045178 IDA
 Experiment description of studies that identified Zasp52 in sEVs
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Zasp52
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Neto  
Two-hybrid Drosophila melanogaster
2 RpS3  
Two-hybrid Drosophila melanogaster
3 CG44085  
Two-hybrid Drosophila melanogaster
4 pot  
Two-hybrid Drosophila melanogaster
5 CG32068  
Two-hybrid Drosophila melanogaster
6 Mal-A2  
Two-hybrid Drosophila melanogaster
7 CG12498  
Two-hybrid Drosophila melanogaster
8 Lasp  
Two-hybrid Drosophila melanogaster
9 CG12470  
Two-hybrid Drosophila melanogaster
10 Ccs  
Two-hybrid Drosophila melanogaster
11 Cpr56F  
Two-hybrid Drosophila melanogaster
12 mod(mdg4)  
Two-hybrid Drosophila melanogaster
13 Tak1  
Affinity Capture-MS Drosophila melanogaster
14 key  
Affinity Capture-MS Drosophila melanogaster
15 CG10306  
Two-hybrid Drosophila melanogaster
16 Iap2  
Affinity Capture-MS Drosophila melanogaster
17 PGRP-LE  
Affinity Capture-MS Drosophila melanogaster
18 CG13040  
Two-hybrid Drosophila melanogaster
19 CG4810  
Two-hybrid Drosophila melanogaster
20 CG18745  
Two-hybrid Drosophila melanogaster
21 rump  
Two-hybrid Drosophila melanogaster
22 Rel  
Affinity Capture-MS Drosophila melanogaster
23 CG8541  
Two-hybrid Drosophila melanogaster
24 ird5  
Affinity Capture-MS Drosophila melanogaster
25 PGRP-LC  
Affinity Capture-MS Drosophila melanogaster
26 Actn  
Two-hybrid Drosophila melanogaster
27 CG12200  
Two-hybrid Drosophila melanogaster
28 Tab2  
Affinity Capture-MS Drosophila melanogaster
29 CG6843  
Two-hybrid Drosophila melanogaster
30 CG5445  
Two-hybrid Drosophila melanogaster
31 CR18854  
Two-hybrid Drosophila melanogaster
32 CenG1A  
Two-hybrid Drosophila melanogaster
View the network image/svg+xml
 Pathways in which Zasp52 is involved
No pathways found





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