Gene description for Btk
Gene name Bruton agammaglobulinemia tyrosine kinase
Gene symbol Btk
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Btk
ExoCarta ExoCarta_367901
Vesiclepedia VP_367901
Entrez Gene 367901
 Btk identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Btk
Molecular Function
    protein tyrosine kinase activity GO:0004713 IDA
    protein tyrosine kinase activity GO:0004713 ISO
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IBA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 ISO
    ATP binding GO:0005524 IEA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISO
    phospholipase activator activity GO:0016004 ISO
    histone H3Y41 kinase activity GO:0035401 IEA
    identical protein binding GO:0042802 ISO
    phospholipase binding GO:0043274 ISO
    metal ion binding GO:0046872 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    neutrophil homeostasis GO:0001780 IMP
    positive regulation of type III hypersensitivity GO:0001805 IMP
    positive regulation of type I hypersensitivity GO:0001812 IMP
    negative regulation of cytokine production GO:0001818 ISO
    adaptive immune response GO:0002250 IBA
    B cell affinity maturation GO:0002344 ISO
    histamine secretion by mast cell GO:0002553 IMP
    positive regulation of immunoglobulin production GO:0002639 IMP
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 ISO
    peptidyl-tyrosine phosphorylation GO:0018108 ISO
    peptidyl-tyrosine phosphorylation GO:0018108 ISO
    proteoglycan catabolic process GO:0030167 IMP
    negative regulation of B cell proliferation GO:0030889 ISO
    positive regulation of B cell proliferation GO:0030890 IMP
    response to lipopolysaccharide GO:0032496 IMP
    negative regulation of interleukin-10 production GO:0032693 IMP
    positive regulation of interleukin-6 production GO:0032755 IMP
    positive regulation of tumor necrosis factor production GO:0032760 IMP
    cellular response to reactive oxygen species GO:0034614 IMP
    intracellular signal transduction GO:0035556 ISO
    peptidyl-tyrosine autophosphorylation GO:0038083 IMP
    protein autophosphorylation GO:0046777 IDA
    cell maturation GO:0048469 ISO
    positive regulation of phagocytosis GO:0050766 IMP
    T cell receptor signaling pathway GO:0050852 IBA
    B cell receptor signaling pathway GO:0050853 IBA
    B cell receptor signaling pathway GO:0050853 ISO
    negative regulation of B cell activation GO:0050869 IMP
    monocyte proliferation GO:0061516 IMP
    negative regulation of leukocyte proliferation GO:0070664 IMP
    cellular response to molecule of fungal origin GO:0071226 IDA
    cellular response to interleukin-7 GO:0098761 ISO
    positive regulation of cGAS/STING signaling pathway GO:0141111 ISO
    positive regulation of interleukin-17A production GO:0150153 IMP
    positive regulation of NLRP3 inflammasome complex assembly GO:1900227 ISO
    positive regulation of synoviocyte proliferation GO:1901647 IMP
    eosinophil homeostasis GO:1990959 IMP
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    cytoplasmic vesicle GO:0031410 ISO
    membrane raft GO:0045121 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified Btk in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Btk
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Btk is involved
No pathways found





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