Gene description for Khc
Gene name Kinesin heavy chain
Gene symbol Khc
Other names/aliases CG7765
DK
DKH
DmK
DmKHC
DmelCG7765
Dmkin
KHC
KIF 5A
KIF5B
KIN
Kin
Kin-1
Kinesin-1
khc
kin
kinesin
kinesin-1
l(2)W12
l(2)k13219
l(2)k13314
l(2R)W12
pgs
Species Drosophila melanogaster
 Database cross references - Khc
ExoCarta ExoCarta_36810
Vesiclepedia VP_36810
Entrez Gene 36810
UniProt P17210  
 Khc identified in sEVs derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Khc
Molecular Function
    cytoskeletal motor activity GO:0003774 NAS
    microtubule motor activity GO:0003777 IDA
    tropomyosin binding GO:0005523 IPI
    microtubule binding GO:0008017 IDA
    plus-end-directed microtubule motor activity GO:0008574 TAS
Biological Process
    eye photoreceptor cell differentiation GO:0001754 IGI
    microtubule-based movement GO:0007018 IDA
    nuclear migration GO:0007097 IMP
    ovarian nurse cell to oocyte transport GO:0007300 TAS
    oocyte dorsal/ventral axis specification GO:0007310 IMP
    pole plasm assembly GO:0007315 IMP
    regulation of pole plasm oskar mRNA localization GO:0007317 IMP
    regulation of pole plasm oskar mRNA localization GO:0007317 TAS
    axonogenesis GO:0007409 IMP
    larval somatic muscle development GO:0007526 IMP
    axo-dendritic transport GO:0008088 IMP
    oocyte microtubule cytoskeleton polarization GO:0008103 IMP
    larval locomotory behavior GO:0008345 IMP
    transport along microtubule GO:0010970 IDA
    stress granule disassembly GO:0035617 IMP
    pole plasm oskar mRNA localization GO:0045451 IMP
    pole plasm oskar mRNA localization GO:0045451 NAS
    microtubule polymerization GO:0046785 IMP
    dorsal appendage formation GO:0046843 IMP
    mitochondrion distribution GO:0048311 IMP
    intracellular distribution of mitochondria GO:0048312 IMP
    synaptic vesicle transport GO:0048489 IGI
    dendrite morphogenesis GO:0048813 IMP
    microtubule sliding GO:0051012 IMP
    centrosome separation GO:0051299 IMP
    actin filament bundle organization GO:0061572 IMP
    anterograde dendritic transport GO:0098937 IMP
    anterograde axonal transport of mitochondrion GO:0098957 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    kinesin complex GO:0005871 IDA
    actin cap GO:0030478 IDA
    microtubule plus-end GO:0035371 IDA
    axon cytoplasm GO:1904115 IDA
 Experiment description of studies that identified Khc in sEVs
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Khc
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Met  
Two-hybrid Drosophila melanogaster
2 Pink1  
Synthetic Rescue Drosophila melanogaster
3 mey  
Two-hybrid Drosophila melanogaster
4 CG34404  
Two-hybrid Drosophila melanogaster
5 CG13474  
Two-hybrid Drosophila melanogaster
6 CG14829  
Two-hybrid Drosophila melanogaster
7 CG13321  
Two-hybrid Drosophila melanogaster
8 cry  
Proximity Label-MS Drosophila melanogaster
9 sina  
Two-hybrid Drosophila melanogaster
10 CG3308  
Two-hybrid Drosophila melanogaster
11 cad  
Two-hybrid Drosophila melanogaster
12 tkv  
Affinity Capture-MS Drosophila melanogaster
13 Klc  
Two-hybrid Drosophila melanogaster
Affinity Capture-MS Drosophila melanogaster
14 mon2  
Two-hybrid Drosophila melanogaster
15 rad50  
Two-hybrid Drosophila melanogaster
16 Cdc5  
Two-hybrid Drosophila melanogaster
17 Ssu72  
Two-hybrid Drosophila melanogaster
18 Exo70  
Two-hybrid Drosophila melanogaster
19 Cyp6t1  
Two-hybrid Drosophila melanogaster
View the network image/svg+xml



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