Gene description for Vps20
Gene name Vacuolar protein sorting 20
Gene symbol Vps20
Other names/aliases CG4071
DmVPS20
DmelCG4071
ESCRT-III
anon-EST:Posey40
vps20
Species Drosophila melanogaster
 Database cross references - Vps20
ExoCarta ExoCarta_37581
Vesiclepedia VP_37581
Entrez Gene 37581
 Vps20 identified in sEVs derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Vps20
Biological Process
    endosome transport via multivesicular body sorting pathway GO:0032509 IMP
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 IMP
Subcellular Localization
    ESCRT III complex GO:0000815 ISS
 Experiment description of studies that identified Vps20 in sEVs
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Vps20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pk92B  
Two-hybrid Drosophila melanogaster
2 sisA  
Two-hybrid Drosophila melanogaster
3 CG8038  
Two-hybrid Drosophila melanogaster
4 CG11902  
Two-hybrid Drosophila melanogaster
5 CG3199  
Two-hybrid Drosophila melanogaster
6 Mio  
Two-hybrid Drosophila melanogaster
7 RpL4  
Two-hybrid Drosophila melanogaster
8 CG14104  
Two-hybrid Drosophila melanogaster
9 dgt2  
Two-hybrid Drosophila melanogaster
10 betaTub56D  
Two-hybrid Drosophila melanogaster
11 CG11164  
Two-hybrid Drosophila melanogaster
12 CG34357  
Two-hybrid Drosophila melanogaster
13 CG11696  
Two-hybrid Drosophila melanogaster
14 Vps25  
Two-hybrid Drosophila melanogaster
Two-hybrid Drosophila melanogaster
15 shrb  
Two-hybrid Drosophila melanogaster
16 CG10904  
Two-hybrid Drosophila melanogaster
17 CG45068  
Two-hybrid Drosophila melanogaster
18 ATPsyn-b  
Two-hybrid Drosophila melanogaster
19 CG6398  
Two-hybrid Drosophila melanogaster
View the network image/svg+xml
 Pathways in which Vps20 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here