Gene description for Wee2
Gene name WEE1 homolog 2 (S. pombe)
Gene symbol Wee2
Other names/aliases Gm1065
Wee1B
Species Mus musculus
 Database cross references - Wee2
ExoCarta ExoCarta_381759
Vesiclepedia VP_381759
Entrez Gene 381759
UniProt Q66JT0  
 Wee2 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Wee2
Molecular Function
    magnesium ion binding GO:0000287 IEA
    protein tyrosine kinase activity GO:0004713 IBA
    protein tyrosine kinase activity GO:0004713 TAS
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IEA
    ATP binding GO:0005524 IEA
    histone H3Y41 kinase activity GO:0035401 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    mitotic cell cycle GO:0000278 IEA
    chromatin remodeling GO:0006338 IEA
    female meiotic nuclear division GO:0007143 IMP
    female pronucleus assembly GO:0035038 IMP
    positive regulation of phosphorylation GO:0042327 IDA
    negative regulation of cyclin-dependent protein serine/threonine kinase activity GO:0045736 IMP
    regulation of meiosis I GO:0060631 IBA
    regulation of meiosis I GO:0060631 IMP
    regulation of fertilization GO:0080154 IMP
    negative regulation of oocyte maturation GO:1900194 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Wee2 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Wee2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Wee2 is involved
No pathways found





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