Gene description for Wee2
Gene name WEE1 homolog 2 (S. pombe)
Gene symbol Wee2
Other names/aliases Gm1065
Wee1B
Species Mus musculus
 Database cross references - Wee2
ExoCarta ExoCarta_381759
Entrez Gene 381759
UniProt Q66JT0  
 Wee2 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Wee2
Molecular Function
    nucleotide binding GO:0000166 IEA
    magnesium ion binding GO:0000287 IEA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IEA
    protein tyrosine kinase activity GO:0004713 TAS
    ATP binding GO:0005524 IEA
    protein kinase activity GO:0004672 IEA
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    transferase activity GO:0016740 IEA
    metal ion binding GO:0046872 IEA
    kinase activity GO:0016301 IEA
Biological Process
    regulation of meiosis I GO:0060631 IMP
    phosphorylation GO:0016310 IEA
    mitotic nuclear division GO:0007067 IEA
    meiotic cell cycle GO:0051321 IEA
    negative regulation of cyclin-dependent protein serine/threonine kinase activity GO:0045736 IMP
    female meiotic division GO:0007143 IMP
    negative regulation of oocyte maturation GO:1900194 IMP
    protein phosphorylation GO:0006468 IEA
    female pronucleus assembly GO:0035038 IMP
    peptidyl-tyrosine phosphorylation GO:0018108 TAS
Subcellular Localization
    cytoplasm GO:0005737 ISO
    nucleoplasm GO:0005654 ISO
    nucleus GO:0005634 IDA
 Experiment description of studies that identified Wee2 in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Wee2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Wee2 is involved
No pathways found





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