Gene description for Galphai
Gene name G protein alpha i subunit
Gene symbol Galphai
Other names/aliases CG10060
DG[[alpha1]]
DGalpha1
DmelCG10060
G-alpha-65A
G-ia65A
G-ialpha65A
G-ialpha65a
G-oalpha65A
G[[alphai]]
G[[i]]
G[[i]]alpha
Gai
Galpha i
Galpha65A
Galpha[[i65A]]
Galpha[[i]]
Galphaiota65A
Gi
Gi-alpha
Gialpha
i
Species Drosophila melanogaster
 Database cross references - Galphai
ExoCarta ExoCarta_38765
Entrez Gene 38765
UniProt P20353  
 Galphai identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Galphai
Molecular Function
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    DNA binding GO:0003677 IDA
    G-protein coupled receptor binding GO:0001664 IBA
    GTPase activity GO:0003924 NAS
    signal transducer activity GO:0004871 IBA
Biological Process
    asymmetric neuroblast division GO:0055059 IMP
    cortical actin cytoskeleton organization GO:0030866 IMP
    asymmetric cell division GO:0008356 IMP
    septate junction assembly GO:0019991 IMP
    asymmetric protein localization involved in cell fate determination GO:0045167 IMP
    establishment of glial blood-brain barrier GO:0060857 IMP
    regulation of protein localization GO:0032880 IMP
    adenylate cyclase-modulating G-protein coupled receptor signaling pathway GO:0007188 IBA
    ventral cord development GO:0007419 IMP
    calcium-mediated signaling GO:0019722 IMP
    establishment of spindle orientation GO:0051294 IMP
    G-protein coupled receptor signaling pathway GO:0007186 NAS
Subcellular Localization
    heterotrimeric G-protein complex GO:0005834 NAS
    cell cortex GO:0005938 TAS
    apical cortex GO:0045179 IDA
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IBA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified Galphai in exosomes
1
Experiment ID 202
ISEV standards
IEM
EV Biophysical techniques
TSG10|RAB35
EV Cytosolic markers
FLOT2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP.
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Galphai
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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