Gene description for RpL18
Gene name Ribosomal protein L18
Gene symbol RpL18
Other names/aliases CG8615
DmelCG8615
Rp L18
RpL18e
Species Drosophila melanogaster
 Database cross references - RpL18
ExoCarta ExoCarta_38794
Vesiclepedia VP_38794
Entrez Gene 38794
UniProt Q9VS34  
 RpL18 identified in sEVs derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for RpL18
Molecular Function
    structural constituent of ribosome GO:0003735 HDA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 TAS
Biological Process
    cytoplasmic translation GO:0002181 IC
    cytoplasmic translation GO:0002181 TAS
Subcellular Localization
    cytosolic large ribosomal subunit GO:0022625 TAS
    cytosolic ribosome GO:0022626 HDA
    cytosolic ribosome GO:0022626 IDA
 Experiment description of studies that identified RpL18 in sEVs
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RpL18
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CG30122  
Two-hybrid Drosophila melanogaster
2 CG9173  
Two-hybrid Drosophila melanogaster
3 CTPsyn  
Proximity Label-MS Drosophila melanogaster
4 Neto  
Two-hybrid Drosophila melanogaster
5 me31B  
Proximity Label-MS Drosophila melanogaster
6 sinah  
Two-hybrid Drosophila melanogaster
7 Not10  
Two-hybrid Drosophila melanogaster
View the network image/svg+xml



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