Gene description for ARHGAP6
Gene name Rho GTPase activating protein 6
Gene symbol ARHGAP6
Other names/aliases RHOGAP6
RHOGAPX-1
Species Homo sapiens
 Database cross references - ARHGAP6
ExoCarta ExoCarta_395
Vesiclepedia VP_395
Entrez Gene 395
HGNC 676
MIM 300118
UniProt O43182  
 ARHGAP6 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ARHGAP6
Molecular Function
    GTPase activator activity GO:0005096 TAS
    phospholipase activator activity GO:0016004 IDA
    SH3 domain binding GO:0017124 IEA
    phospholipase binding GO:0043274 IPI
Biological Process
    actin filament organization GO:0007015 IBA
    Rho protein signal transduction GO:0007266 NAS
    positive regulation of phospholipase activity GO:0010518 IDA
    actin filament polymerization GO:0030041 NAS
    focal adhesion assembly GO:0048041 IBA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    negative regulation of stress fiber assembly GO:0051497 IMP
    negative regulation of focal adhesion assembly GO:0051895 IMP
Subcellular Localization
    cytoplasm GO:0005737 IMP
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    actin filament GO:0005884 NAS
    actin cytoskeleton GO:0015629 ISS
 Experiment description of studies that identified ARHGAP6 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARHGAP6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LNX1  
Two-hybrid Homo sapiens
2 Arhgap30  
Affinity Capture-MS Mus musculus
3 Arhgap28  
Affinity Capture-MS Mus musculus
4 Arhgap18  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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