Gene description for ACTB
Gene name actin beta
Gene symbol ACTB
Other names/aliases -
Species Canis lupus familiaris
 Database cross references - ACTB
ExoCarta ExoCarta_403580
Vesiclepedia VP_403580
Entrez Gene 403580
UniProt -   
 ACTB identified in sEVs derived from the following tissue/cell type
Kidney cells 33638284    
Kidney cells 33638284    
 Gene ontology annotations for ACTB
 Experiment description of studies that identified ACTB in sEVs
1
Experiment ID 393
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33638284    
Organism Canis lupus familiaris
Experiment description "Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition"
Authors "Shafiq A, Suwakulsiri W, Rai A, Chen M, Greening DW, Zhu HJ, Xu R, Simpson RJ."
Journal name Proteomics
Publication year 2021
Sample Kidney cells
Sample name MDCK - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 394
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33638284    
Organism Canis lupus familiaris
Experiment description "Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition"
Authors "Shafiq A, Suwakulsiri W, Rai A, Chen M, Greening DW, Zhu HJ, Xu R, Simpson RJ."
Journal name Proteomics
Publication year 2021
Sample Kidney cells
Sample name MDCK (21D1) - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
 Protein-protein interactions for ACTB
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ACTB is involved
PathwayEvidenceSource
Axon guidance IEA Reactome
B-WICH complex positively regulates rRNA expression IEA Reactome
Cell junction organization IEA Reactome
Cell-Cell communication IEA Reactome
Cell-extracellular matrix interactions IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Deubiquitination IEA Reactome
Developmental Biology IEA Reactome
DNA Damage Recognition in GG-NER IEA Reactome
DNA Repair IEA Reactome
EPH-ephrin mediated repulsion of cells IEA Reactome
EPH-Ephrin signaling IEA Reactome
EPHB-mediated forward signaling IEA Reactome
Epigenetic regulation of gene expression IEA Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
Formation of annular gap junctions IEA Reactome
Gap junction degradation IEA Reactome
Gap junction trafficking IEA Reactome
Gap junction trafficking and regulation IEA Reactome
Gene expression (Transcription) IEA Reactome
Global Genome Nucleotide Excision Repair (GG-NER) IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
MAP2K and MAPK activation IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
Membrane Trafficking IEA Reactome
Metabolism of proteins IEA Reactome
Nervous system development IEA Reactome
Nucleotide Excision Repair IEA Reactome
Positive epigenetic regulation of rRNA expression IEA Reactome
Post-translational protein modification IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
RHO GTPase cycle IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
RHO GTPases activate IQGAPs IEA Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
RHOF GTPase cycle IEA Reactome
Signal Transduction IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
UCH proteinases IEA Reactome
Vesicle-mediated transport IEA Reactome





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