Experiment ID |
202 |
ISEV standards |
✔
IEM
|
EV Biophysical techniques |
✔
TSG10|RAB35
|
EV Cytosolic markers |
✔
FLOT2
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23035643
|
Organism |
Drosophila melanogaster |
Experiment description |
Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes. |
Authors |
Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP. |
Journal name |
Traffic
|
Publication year |
2012 |
Sample |
Drosophila S2 cells |
Sample name |
Normal-Drosophila S2 tub-Wg |
Isolation/purification methods |
Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |