Gene description for Clic1
Gene name chloride intracellular channel 1
Gene symbol Clic1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Clic1
ExoCarta ExoCarta_406864
Vesiclepedia VP_406864
Entrez Gene 406864
UniProt Q6MG61  
 Clic1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Clic1
Molecular Function
    voltage-gated chloride channel activity GO:0005247 TAS
    chloride channel activity GO:0005254 IBA
    chloride channel activity GO:0005254 IEA
    chloride channel activity GO:0005254 ISO
    oxidoreductase activity GO:0016491 IEA
Biological Process
    chloride transport GO:0006821 IBA
    chloride transport GO:0006821 ISO
    chloride transport GO:0006821 TAS
    positive regulation of osteoblast differentiation GO:0045669 IEA
    positive regulation of osteoblast differentiation GO:0045669 ISO
    regulation of mitochondrial membrane potential GO:0051881 IEA
    regulation of mitochondrial membrane potential GO:0051881 ISO
    platelet aggregation GO:0070527 ISO
    chloride transmembrane transport GO:1902476 IEA
Subcellular Localization
    nucleus GO:0005634 ISO
    nuclear envelope GO:0005635 ISO
    nuclear envelope GO:0005635 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    endoplasmic reticulum GO:0005783 IEA
    plasma membrane GO:0005886 IEA
    membrane GO:0016020 IBA
    nuclear membrane GO:0031965 IEA
    mitochondrial membrane GO:0031966 IDA
    chloride channel complex GO:0034707 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Clic1 in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Clic1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Clic1 is involved
No pathways found





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