ExoCarta: Gene summary

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Gene description for RT1-T24-4
Gene name	RT1 class I, locus T24, gene 4
Gene symbol	RT1-T24-4
Other names/aliases	H2-T24 RT1-149
Species	Rattus norvegicus
Database cross references - RT1-T24-4
ExoCarta	ExoCarta_414784
Entrez Gene	414784
RT1-T24-4 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for RT1-T24-4

Molecular Function
	peptide antigen binding	GO:0042605	IBA
	receptor binding	GO:0005102	IBA
Biological Process
	immune response	GO:0006955	IEA
	antigen processing and presentation of peptide antigen via MHC class I	GO:0002474	IBA
Subcellular Localization
	integral component of membrane	GO:0016021	IEA
	plasma membrane	GO:0005886	IBA
	MHC class I protein complex	GO:0042612	IEA

Experiment description of studies that identified RT1-T24-4 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ HSC70\|HSP90\|TSG101	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting