Gene description for MMP12
Gene name matrix metallopeptidase 12
Gene symbol MMP12
Other names/aliases HME
ME
MME
MMP-12
Species Homo sapiens
 Database cross references - MMP12
ExoCarta ExoCarta_4321
Vesiclepedia VP_4321
Entrez Gene 4321
HGNC 7158
MIM 601046
UniProt P39900  
 MMP12 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MMP12
Molecular Function
    core promoter sequence-specific DNA binding GO:0001046 IMP
    endopeptidase activity GO:0004175 TAS
    metalloendopeptidase activity GO:0004222 IBA
    metalloendopeptidase activity GO:0004222 IDA
    metalloendopeptidase activity GO:0004222 IMP
    metalloendopeptidase activity GO:0004222 TAS
    serine-type endopeptidase activity GO:0004252 TAS
    calcium ion binding GO:0005509 IMP
    collagen binding GO:0005518 IPI
    zinc ion binding GO:0008270 IMP
    sequence-specific DNA binding GO:0043565 IMP
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    proteolysis GO:0006508 IDA
    proteolysis GO:0006508 IMP
    protein import into nucleus GO:0006606 IMP
    extracellular matrix disassembly GO:0022617 TAS
    extracellular matrix organization GO:0030198 IBA
    collagen catabolic process GO:0030574 IBA
    collagen catabolic process GO:0030574 TAS
    positive regulation of interferon-alpha production GO:0032727 IEA
    wound healing, spreading of epidermal cells GO:0035313 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    lung alveolus development GO:0048286 IEA
    regulation of defense response to virus by host GO:0050691 IEA
    positive regulation of epithelial cell proliferation involved in wound healing GO:0060054 IDA
    elastin catabolic process GO:0060309 IDA
    negative regulation of type I interferon-mediated signaling pathway GO:0060339 IEA
    positive regulation of type I interferon-mediated signaling pathway GO:0060340 IEA
    bronchiole development GO:0060435 IEA
    cellular response to virus GO:0098586 IEA
    response to amyloid-beta GO:1904645 TAS
    negative regulation of endothelial cell-matrix adhesion via fibronectin GO:1904905 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IMP
    nucleus GO:0005634 IMP
    cytoplasm GO:0005737 IDA
    extracellular matrix GO:0031012 IEA
 Experiment description of studies that identified MMP12 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MMP12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 SARAF  
Affinity Capture-MS Homo sapiens
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