Gene description for ALiX
Gene name ALG-2 interacting protein X
Gene symbol ALiX
Other names/aliases AIP1
ALIX
Aip1
Alix
CG12876
DAIP1
DmelCG12876
Snf7
Species Drosophila melanogaster
 Database cross references - ALiX
ExoCarta ExoCarta_43330
Entrez Gene 43330
 ALiX identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for ALiX
Molecular Function
    ubiquitin-specific protease activity GO:0004843 ISS
    signal transducer activity GO:0004871 ISS
Biological Process
    exosomal secretion GO:1990182 IMP
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISS
    female germ-line stem cell asymmetric division GO:0048132 IMP
    actin filament polymerization GO:0030041 IMP
    positive regulation of JNK cascade GO:0046330 NAS
    apoptotic process GO:0006915 NAS
    mitotic cytokinesis GO:0000281 IMP
    male germ-line stem cell asymmetric division GO:0048133 IMP
Subcellular Localization
    contractile ring GO:0070938 IDA
    centrosome GO:0005813 IDA
    endosome GO:0005768 ISS
    midbody GO:0030496 IDA
    intercellular bridge GO:0045171 IDA
 Experiment description of studies that identified ALiX in exosomes
1
Experiment ID 202
ISEV standards
IEM
EV Biophysical techniques
TSG10|RAB35
EV Cytosolic markers
FLOT2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP.
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ALiX
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ALiX is involved
No pathways found





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