Gene description for Dnmt3b
Gene name DNA (cytosine-5-)-methyltransferase 3 beta
Gene symbol Dnmt3b
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Dnmt3b
ExoCarta ExoCarta_444985
Vesiclepedia VP_444985
Entrez Gene 444985
 Dnmt3b identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Dnmt3b
Molecular Function
    DNA binding GO:0003677 IBA
    DNA binding GO:0003677 ISO
    chromatin binding GO:0003682 IDA
    transcription corepressor activity GO:0003714 ISO
    DNA (cytosine-5-)-methyltransferase activity GO:0003886 ISO
    protein binding GO:0005515 IPI
    methyltransferase activity GO:0008168 ISO
    metal ion binding GO:0046872 IEA
    DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates GO:0051718 IBA
    DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates GO:0051718 ISO
    DNA (cytosine-5-)-methyltransferase activity, acting on CpN substrates GO:0051719 IEA
    DNA (cytosine-5-)-methyltransferase activity, acting on CpNpG substrates GO:0051720 IEA
    lncRNA binding GO:0106222 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IBA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    response to hypoxia GO:0001666 IEP
    DNA methylation-dependent constitutive heterochromatin formation GO:0006346 ISO
    response to xenobiotic stimulus GO:0009410 IEP
    response to toxic substance GO:0009636 IEP
    response to ionizing radiation GO:0010212 IEP
    positive regulation of gene expression GO:0010628 ISO
    response to activity GO:0014823 IEP
    response to caffeine GO:0031000 IEP
    protein-containing complex localization GO:0031503 ISO
    heterochromatin formation GO:0031507 ISO
    methylation GO:0032259 IEA
    response to estradiol GO:0032355 IEP
    response to vitamin A GO:0033189 IEP
    epigenetic regulation of gene expression GO:0040029 ISO
    response to cocaine GO:0042220 IEP
    epigenetic programming of gene expression GO:0043045 ISO
    negative regulation of gene expression, epigenetic GO:0045814 ISO
    cellular response to amino acid stimulus GO:0071230 ISO
    cellular response to hyperoxia GO:0071455 IEP
    cellular response to dexamethasone stimulus GO:0071549 IEP
    response to alcohol GO:0097305 IEP
    transposable element silencing by heterochromatin formation GO:0141005 ISO
    autosome genomic imprinting GO:0141068 ISO
    gene silencing by piRNA-directed DNA methylation GO:0141176 ISO
Subcellular Localization
    chromosome, centromeric region GO:0000775 ISO
    heterochromatin GO:0000792 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    catalytic complex GO:1902494 ISO
 Experiment description of studies that identified Dnmt3b in sEVs
1
Experiment ID 92
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|GAPDH|CD9|CD151
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|GAPDH|CD9|CD151
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Dnmt3b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hdac2  
Affinity Capture-Western Rattus norvegicus
Co-fractionation Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Co-fractionation Rattus norvegicus
View the network image/svg+xml
 Pathways in which Dnmt3b is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here