Gene ontology annotations for POTEE
Experiment description of studies that identified POTEE in exosomes
1
Experiment ID
21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
19837982
Organism
Homo sapiens
Experiment description
Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors
"Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name
MCP
Publication year
2009
Sample
Colorectal cancer cells
Sample name
LIM1215
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.10-1.12 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [Orbitrap] Western blotting
2
Experiment ID
207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DKO-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
3
Experiment ID
208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
Dks-8
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
4
Experiment ID
209
MISEV standards
✘
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DLD-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
5
Experiment ID
1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
34887515
Organism
Homo sapiens
Experiment description
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Colorectal cancer cells
Sample name
DiFi
Isolation/purification methods
Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting Mass spectrometry RNA sequencing
6
Experiment ID
224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
7
Experiment ID
211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
8
Experiment ID
212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
9
Experiment ID
275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel sensitive
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.12-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry/Flow cytometry/Western blotting
10
Experiment ID
191
MISEV standards
✘
Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20124223
Organism
Homo sapiens
Experiment description
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors
"Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name
Mol Cell Proteomics
Publication year
2010
Sample
Squamous carcinoma cells
Sample name
Squamous carcinoma cell (A431)
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
11
Experiment ID
217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
12
Experiment ID
193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
"Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
13
Experiment ID
194
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
"Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Patients of basement membrane nephropathy
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
14
Experiment ID
195
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
"Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Patients of early IgA nephropathy
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
15
Experiment ID
196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22418980
Organism
Homo sapiens
Experiment description
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors
"Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name
Kidney Int
Publication year
2012
Sample
Urine
Sample name
Urine - Normal high density
Isolation/purification methods
Differential centrifugation Sucrose cushion
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
16
Experiment ID
197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22418980
Organism
Homo sapiens
Experiment description
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors
"Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name
Kidney Int
Publication year
2012
Sample
Urine
Sample name
Urine - Normal low density
Isolation/purification methods
Differential centrifugation Sucrose cushion
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for POTEE
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
SLFN11
91607
Proximity Label-MS
Homo sapiens
2
ARHGEF25
115557
Affinity Capture-MS
Homo sapiens
3
ECT2L
Affinity Capture-MS
Homo sapiens
4
MTCH1
23787
Proximity Label-MS
Homo sapiens
5
FBXL4
26235
Proximity Label-MS
Homo sapiens
6
FSTL4
Affinity Capture-MS
Homo sapiens
7
LOXL3
84695
Affinity Capture-MS
Homo sapiens
8
FMR1
2332
Affinity Capture-MS
Homo sapiens
9
SP1
Affinity Capture-MS
Homo sapiens
10
AP4B1
Affinity Capture-MS
Homo sapiens
11
HIST1H2BG
8339
Affinity Capture-MS
Homo sapiens
12
ACTC1
70
Cross-Linking-MS (XL-MS)
Homo sapiens
13
FGD4
121512
Affinity Capture-MS
Homo sapiens
14
KIF20A
10112
Affinity Capture-MS
Homo sapiens
15
TMEM186
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
16
RPUSD4
84881
Proximity Label-MS
Homo sapiens
17
RLN2
Affinity Capture-MS
Homo sapiens
18
CST7
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
19
DEGS1
Affinity Capture-MS
Homo sapiens
20
EFNA3
Affinity Capture-MS
Homo sapiens
21
TM2D3
Affinity Capture-MS
Homo sapiens
22
KLK10
5655
Affinity Capture-MS
Homo sapiens
23
FAM150B
Affinity Capture-MS
Homo sapiens
24
PAK4
10298
Affinity Capture-MS
Homo sapiens
25
KIF23
9493
Affinity Capture-MS
Homo sapiens
26
EXOC1
55763
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
27
CCR2
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
28
CXADR
1525
Proximity Label-MS
Homo sapiens
29
ACTG2
72
Cross-Linking-MS (XL-MS)
Homo sapiens
30
CALD1
800
Affinity Capture-MS
Homo sapiens
31
CPA5
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
32
KLRC1
Affinity Capture-MS
Homo sapiens
33
OBSL1
23363
Affinity Capture-MS
Homo sapiens
34
LAMTOR3
8649
Affinity Capture-MS
Homo sapiens
35
BMI1
Affinity Capture-MS
Homo sapiens
36
UCHL5
51377
Affinity Capture-MS
Homo sapiens
37
PRTN3
5657
Affinity Capture-MS
Homo sapiens
38
TFCP2
7024
Affinity Capture-MS
Homo sapiens
39
FASTKD2
Proximity Label-MS
Homo sapiens
40
KIF14
9928
Affinity Capture-MS
Homo sapiens
41
CINP
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
42
WDR1
9948
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
43
ACTA2
59
Cross-Linking-MS (XL-MS)
Homo sapiens
44
VAV1
7409
Affinity Capture-MS
Homo sapiens
45
POTEI
653269
Cross-Linking-MS (XL-MS)
Homo sapiens
46
CHST14
113189
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
47
ARHGEF11
9826
Affinity Capture-MS
Homo sapiens
48
ERBB3
2065
Affinity Capture-MS
Homo sapiens
49
CLSTN1
22883
Affinity Capture-MS
Homo sapiens
50
GPR35
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
51
CHMP4B
128866
Affinity Capture-MS
Homo sapiens
52
YAP1
10413
Affinity Capture-MS
Homo sapiens
53
FIBIN
Affinity Capture-MS
Homo sapiens
54
IL27RA
9466
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
55
DHFRL1
Proximity Label-MS
Homo sapiens
56
TRIM72
Affinity Capture-MS
Homo sapiens
57
SDF2L1
23753
Affinity Capture-MS
Homo sapiens
58
ACTG1
71
Affinity Capture-MS
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
59
ANLN
54443
Affinity Capture-MS
Homo sapiens
60
OTC
5009
Proximity Label-MS
Homo sapiens
61
FANCD2
Affinity Capture-MS
Homo sapiens
62
CDC73
Affinity Capture-MS
Homo sapiens
63
RC3H2
Affinity Capture-MS
Homo sapiens
64
ECT2
1894
Affinity Capture-MS
Homo sapiens
65
OCRL
4952
Affinity Capture-MS
Homo sapiens
66
HMOX2
3163
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
67
PRG2
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
68
ACTBL2
345651
Cross-Linking-MS (XL-MS)
Homo sapiens
69
IFNE
Affinity Capture-MS
Homo sapiens
70
ELSPBP1
Affinity Capture-MS
Homo sapiens
71
FKBP5
2289
Affinity Capture-MS
Homo sapiens
72
MED10
Affinity Capture-MS
Homo sapiens
73
ARHGAP25
Affinity Capture-MS
Homo sapiens
74
IL12RB1
Affinity Capture-MS
Homo sapiens
75
NAAA
Affinity Capture-MS
Homo sapiens
76
ACTB
60
Affinity Capture-MS
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
77
KLK9
Affinity Capture-MS
Homo sapiens
78
PQLC2L
Affinity Capture-MS
Homo sapiens
79
PLEKHG1
Affinity Capture-MS
Homo sapiens
80
RPIA
22934
Affinity Capture-MS
Homo sapiens
81
MOGAT1
Affinity Capture-MS
Homo sapiens
82
PLEKHG3
26030
Affinity Capture-MS
Homo sapiens
83
NEUROG3
Affinity Capture-MS
Homo sapiens
84
TGDS
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
85
AKAP1
8165
Proximity Label-MS
Homo sapiens
86
RELA
5970
Affinity Capture-MS
Homo sapiens
87
PHB2
11331
Proximity Label-MS
Homo sapiens
88
ACP1
52
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
89
TERF2
Affinity Capture-MS
Homo sapiens
90
PDGFD
80310
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
91
CHTOP
Affinity Capture-MS
Homo sapiens
92
HMGB1
3146
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
93
C5orf24
134553
Affinity Capture-MS
Homo sapiens
94
PRPH2
5961
Affinity Capture-MS
Homo sapiens
95
LRRCC1
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
96
POTEJ
653781
Cross-Linking-MS (XL-MS)
Homo sapiens
97
CLEC2D
Affinity Capture-MS
Homo sapiens
98
Arhgap1
228359
Affinity Capture-MS
Mus musculus
99
SMURF1
57154
Affinity Capture-MS
Homo sapiens
100
GBA
2629
Affinity Capture-MS
Homo sapiens
101
ZNF684
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
102
ZC2HC1B
Affinity Capture-MS
Homo sapiens
103
PRAME
Affinity Capture-MS
Homo sapiens
104
ACTA1
58
Cross-Linking-MS (XL-MS)
Homo sapiens
105
IGF2BP3
10643
Affinity Capture-MS
Homo sapiens
106
BRICD5
Affinity Capture-MS
Homo sapiens
107
EZH2
Affinity Capture-MS
Homo sapiens
108
ARHGAP36
Affinity Capture-MS
Homo sapiens
109
ERRFI1
54206
Affinity Capture-MS
Homo sapiens
110
RPUSD3
Proximity Label-MS
Homo sapiens
111
DEFA6
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
112
CST11
Affinity Capture-MS
Homo sapiens
113
PMM1
5372
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which POTEE is involved