Gene description for MUC4
Gene name mucin 4, cell surface associated
Gene symbol MUC4
Other names/aliases ASGP
HSA276359
MUC-4
Species Homo sapiens
 Database cross references - MUC4
ExoCarta ExoCarta_4585
Entrez Gene 4585
HGNC 7514
MIM 158372
UniProt Q99102  
 MUC4 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Saliva 19199708    
Squamous carcinoma cells 20124223    
Tracheobronchial cells 19190083    
 Gene ontology annotations for MUC4
Molecular Function
    ErbB-2 class receptor binding GO:0005176 TAS
    extracellular matrix constituent, lubricant activity GO:0030197 NAS
Biological Process
    cell-matrix adhesion GO:0007160 IEA
    maintenance of gastrointestinal epithelium GO:0030277 IMP
    protein O-linked glycosylation GO:0006493 TAS
    O-glycan processing GO:0016266 TAS
    post-translational protein modification GO:0043687 TAS
    cellular protein metabolic process GO:0044267 TAS
Subcellular Localization
    membrane GO:0016020 NAS
    proteinaceous extracellular matrix GO:0005578 NAS
    extracellular space GO:0005615 IDA
    Golgi lumen GO:0005796 TAS
    extracellular exosome GO:0070062 IDA
    vesicle GO:0031982 IDA
    integral component of plasma membrane GO:0005887 TAS
 Experiment description of studies that identified MUC4 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 34
ISEV standards
EM|IEM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD63|MHCI|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
Mass spectrometry
FACS
PubMed ID 19190083    
Organism Homo sapiens
Experiment description Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
Authors Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK
Journal name FASEB
Publication year 2009
Sample Tracheobronchial cells
Sample name Tracheobronchial epithelial cells
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.16 -1.18 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
FACS
 Protein-protein interactions for MUC4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which MUC4 is involved
PathwayEvidenceSource
O-linked glycosylation of mucins TAS Reactome
Termination of O-glycan biosynthesis TAS Reactome





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