Experiment description of studies that identified TSG101 in sEVs

1 Experiment ID 393 MISEV standards ✔ EM Biophysical techniques ✔ CD63|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 33638284     Organism Canis lupus familiaris Experiment description "Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition" Authors "Shafiq A, Suwakulsiri W, Rai A, Chen M, Greening DW, Zhu HJ, Xu R, Simpson RJ." Journal name Proteomics Publication year 2021 Sample Kidney cells Sample name MDCK - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

2 Experiment ID 394 MISEV standards ✔ EM Biophysical techniques ✔ CD63|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 33638284     Organism Canis lupus familiaris Experiment description "Transglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition" Authors "Shafiq A, Suwakulsiri W, Rai A, Chen M, Greening DW, Zhu HJ, Xu R, Simpson RJ." Journal name Proteomics Publication year 2021 Sample Kidney cells Sample name MDCK (21D1) - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

3 Experiment ID 765 MISEV standards ✘ Biophysical techniques ✔ CD9|TSG101|CD63 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - apical Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

4 Experiment ID 766 MISEV standards ✘ Biophysical techniques ✔ CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - apical Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

5 Experiment ID 767 MISEV standards ✘ Biophysical techniques ✔ CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - apical Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

6 Experiment ID 768 MISEV standards ✘ Biophysical techniques ✔ CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - apical Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

7 Experiment ID 769 MISEV standards ✘ Biophysical techniques ✔ CD9|TSG101|CD63 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - basolateral Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

8 Experiment ID 770 MISEV standards ✘ Biophysical techniques ✔ CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - basolateral Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

9 Experiment ID 771 MISEV standards ✘ Biophysical techniques ✔ CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - basolateral Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

10 Experiment ID 772 MISEV standards ✘ Biophysical techniques ✔ CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - basolateral Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

11 Experiment ID 781 MISEV standards ✘ Biophysical techniques ✔ CD63|CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - apical Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

12 Experiment ID 782 MISEV standards ✘ Biophysical techniques ✔ CD63|CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - apical Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

13 Experiment ID 783 MISEV standards ✘ Biophysical techniques ✔ CD63|CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - apical Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

14 Experiment ID 784 MISEV standards ✘ Biophysical techniques ✔ CD63|CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - apical Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

15 Experiment ID 785 MISEV standards ✘ Biophysical techniques ✔ CD63|CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - basolateral Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

16 Experiment ID 786 MISEV standards ✘ Biophysical techniques ✔ CD63|CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - basolateral Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

17 Experiment ID 787 MISEV standards ✘ Biophysical techniques ✔ CD63|CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - basolateral Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

18 Experiment ID 788 MISEV standards ✘ Biophysical techniques ✔ CD63|CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Homo sapiens Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - basolateral Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

19 Experiment ID 817 MISEV standards ✘ Biophysical techniques ✔ CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - apical Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

20 Experiment ID 818 MISEV standards ✘ Biophysical techniques ✔ CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - apical Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

21 Experiment ID 819 MISEV standards ✘ Biophysical techniques ✔ CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - basolateral Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

22 Experiment ID 820 MISEV standards ✘ Biophysical techniques ✔ CD9|TSG101 Enriched markers ✔ HSP90AA1 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 35649370     Organism Canis lupus familiaris Experiment description Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells Authors "Matsui T, Sakamaki Y, Nakashima S, Fukuda M." Journal name Cell Rep Publication year 2022 Sample Kidney epithelial cells Sample name MDCK II - basolateral Isolation/purification methods Differential centrifugation Filtration Immunoaffinity capture Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

Protein-protein interactions for TSG101

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which TSG101 is involved

No pathways found