ExoCarta: Gene summary

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Gene description for Capza2
Gene name	capping protein (actin filament) muscle Z-line, alpha 2
Gene symbol	Capza2
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Capza2
ExoCarta	ExoCarta_493810
Entrez Gene	493810
UniProt	Q3T1K5
Capza2 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Capza2

Molecular Function
	actin binding	GO:0003779	IEA
Biological Process
	barbed-end actin filament capping	GO:0051016	IEA
Subcellular Localization
	cortical cytoskeleton	GO:0030863	ISO
	membrane	GO:0016020	ISO
	F-actin capping protein complex	GO:0008290	ISO
	extracellular exosome	GO:0070062	ISO
	brush border	GO:0005903	ISO

Experiment description of studies that identified Capza2 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting