Gene description for Trim25
Gene name tripartite motif-containing 25
Gene symbol Trim25
Other names/aliases Efp
Znf147
Species Rattus norvegicus
 Database cross references - Trim25
ExoCarta ExoCarta_494338
Vesiclepedia VP_494338
Entrez Gene 494338
 Trim25 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Trim25
Molecular Function
    transcription coactivator activity GO:0003713 ISO
    ubiquitin-protein transferase activity GO:0004842 ISO
    RIG-I binding GO:0039552 ISO
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 ISO
Biological Process
    cytoplasmic pattern recognition receptor signaling pathway GO:0002753 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    protein monoubiquitination GO:0006513 ISO
    response to oxidative stress GO:0006979 ISO
    viral release from host cell GO:0019076 ISO
    regulation of protein localization GO:0032880 ISO
    response to vitamin D GO:0033280 IEP
    ERAD pathway GO:0036503 ISO
    RIG-I signaling pathway GO:0039529 ISO
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISO
    response to estrogen GO:0043627 IEP
    response to estrogen GO:0043627 ISO
    suppression of viral release by host GO:0044790 ISO
    innate immune response GO:0045087 ISO
    positive regulation of DNA-templated transcription GO:0045893 IEA
    regulation of viral entry into host cell GO:0046596 ISO
    negative regulation of viral entry into host cell GO:0046597 ISO
    positive regulation of DNA-binding transcription factor activity GO:0051091 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    protein K48-linked ubiquitination GO:0070936 ISO
    antiviral innate immune response GO:0140374 ISO
    cellular response to leukemia inhibitory factor GO:1990830 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytoplasmic stress granule GO:0010494 ISO
    nuclear body GO:0016604 IEA
    nuclear body GO:0016604 ISO
 Experiment description of studies that identified Trim25 in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Trim25
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Trim25 is involved
No pathways found





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