Gene description for Srsf2
Gene name serine/arginine-rich splicing factor 2
Gene symbol Srsf2
Other names/aliases Sfrs2
Species Rattus norvegicus
 Database cross references - Srsf2
ExoCarta ExoCarta_494445
Vesiclepedia VP_494445
Entrez Gene 494445
UniProt Q6PDU1  
 Srsf2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Srsf2
Molecular Function
    RNA binding GO:0003723 IBA
    protein kinase C binding GO:0005080 IPI
    pre-mRNA binding GO:0036002 ISO
Biological Process
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IBA
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 ISO
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    response to vitamin E GO:0033197 IEP
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    spliceosomal complex GO:0005681 ISO
    perichromatin fibrils GO:0005726 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    PML body GO:0016605 ISO
    nuclear speck GO:0016607 IBA
    nuclear speck GO:0016607 IDA
    nuclear speck GO:0016607 ISO
    interchromatin granule GO:0035061 IDA
 Experiment description of studies that identified Srsf2 in sEVs
1
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Srsf2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Snca  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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