ExoCarta: Gene summary

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Gene description for Srsf2
Gene name	serine/arginine-rich splicing factor 2
Gene symbol	Srsf2
Other names/aliases	Sfrs2
Species	Rattus norvegicus
Database cross references - Srsf2
ExoCarta	ExoCarta_494445
Entrez Gene	494445
UniProt	Q6PDU1
Srsf2 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Srsf2

Molecular Function
	nucleotide binding	GO:0000166	IEA
	pre-mRNA binding	GO:0036002	ISO
	poly(A) RNA binding	GO:0044822	ISO
Biological Process
	regulation of alternative mRNA splicing, via spliceosome	GO:0000381	ISO
	mRNA processing	GO:0006397	IEA
	RNA splicing	GO:0008380	IEA
Subcellular Localization
	PML body	GO:0016605	ISO
	nucleoplasm	GO:0005654	ISO
	nuclear speck	GO:0016607	ISO
	nucleus	GO:0005634	ISO
	extracellular exosome	GO:0070062	ISO
	cytoplasm	GO:0005737	ISO
	spliceosomal complex	GO:0005681	ISO

Experiment description of studies that identified Srsf2 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting