ExoCarta: Gene summary

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Gene description for Amy2a3
Gene name	amylase 2a3
Gene symbol	Amy2a3
Other names/aliases	Amy2
Species	Rattus norvegicus
Database cross references - Amy2a3
ExoCarta	ExoCarta_497039
Vesiclepedia	VP_497039
Entrez Gene	497039
UniProt	P00689
Amy2a3 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Amy2a3

Molecular Function
	calcium ion binding	GO:0005509	ISS
	alpha-amylase activity	GO:0004556	ISS
	protein binding	GO:0005515	IPI
	chloride ion binding	GO:0031404	ISS
	cation binding	GO:0043169	IEA
Biological Process
	carbohydrate metabolic process	GO:0005975	IEA
	carbohydrate catabolic process	GO:0016052	ISS
Subcellular Localization
	extracellular space	GO:0005615	ISS

Experiment description of studies that identified Amy2a3 in exosomes

Experiment ID

MISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70\|MHCI	EV Enriched markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D7 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.25 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting